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:: Volume 13, Issue 2 ( Apr-Jun 2026) ::
Nutr Food Health Dis 2026, 13(2): 9-17 Back to browse issues page
Effects of Tetrasodium Pyrophosphate and Whey Protein Concentrate on Quantitative and Qualitative Characteristics of Gluten-free Breads
Amirhassan Ramezani , Reza Farahmandfar *
PhD, Professor (Associate) at Sari Agricultural Sciences and Natural Resources University, Sari, Iran , re.farahmandfar@gmail.com
Abstract:   (289 Views)
Background and Objectives: Gluten-free breads are essential for individuals with celiac disease and gluten-linked disorders; however, the absence of gluten commonly results in poor texture, low loaf volume and decreased sensory quality. This study aimed to assess the synergistic effects of tetrasodium pyrophosphate and whey protein concentrate on the physicochemical, textural and sensory characteristics of gluten-free breads.
Materials and Methods: Gluten-free bread samples were produced by partially substituting base flours with whey protein concentrate at 0, 10, 15 and 20% (w/w) and incorporating tetrasodium pyrophosphate at concentrations of 0, 0.2 and 0.4% (w/w). Bread quality was assessed through measurements of specific volume, moisture content, baking weight loss, protein content, color parameters, texture profile analysis and sensory evaluation. Data were analyzed using ANOVA and Duncan’s multiple range test at p < 0.05.
Results: Increasing whey protein concentrate levels significantly improved protein and moisture content, yellowness (b*), firmness and specific volume, while decreasing baking weight loss and lightness (l*). Sensory analysis showed that the formulation containing 0.4% tetrasodium pyrophosphate and 15% whey protein concentrate received the highest overall acceptability from the panelists.
Conclusions: WPC and TSPP synergistically improved the specific volume, protein content, and firmness of gluten-free bread while reducing baking weight loss, with the 15% WPC–0.4% TSPP formulation achieving the highest overall sensory acceptability, indicating its potential for industrial application.
 
Keywords: Gluten-free, Whey protein concentrate, Tetrasodium pyrophosphate, Bread fortification, Textural quality, Functional characteristics
Full-Text [PDF 788 kb]   (110 Downloads)    
Article type: Research | Subject: Food Science
Received: 2025/11/5 | Accepted: 2026/02/17 | Published: 2026/09/23

  • Whey protein concentrate significantly increased protein content, moisture retention, firmness and specific volume of gluten-free breads.
  • Tetrasodium pyrophosphate improved gas retention and decreased baking weight loss, particularly at 0.4% concentration.
  • The combination of 15% whey protein concentrate and 0.4% tetrasodium pyrophosphate received the highest sensory acceptability from the panelists.
  • Structural, physicochemical and sensory quality of gluten-free bread were significantly enhanced.

References
1. Alishahi F, Fazel M. Effect of the Nigella sativa Linn concentrate addition on the physical and quality characteristics of gluten-free toast bread during storage. Journal of food science and technology (Iran). 2019 Sep 10;16(91):197-211.
2. Payan R. Introduction to technology of cereal products. Aige Pub. Iran. 2006:420.
3. Biesiekierski JR, Iven J. Non-coeliac gluten sensitivity: piecing the puzzle together. United European gastroenterology journal. 2015 Apr;3(2):160-5. [DOI:10.1177/2050640615578388]
4. Leonard MM, Sapone A, Catassi C, Fasano A. Celiac disease and nonceliac gluten sensitivity: a review. Jama. 2017 Aug 15;318(7):647-56. [DOI:10.1001/jama.2017.9730]
5. Salmi T, Hervonen K, Reunala T. Dermatitis herpetiformis-a cutaneous manifestation of coeliac disease. In: Coeliac Disease and Gluten-Linked Disorders. 2022. p. 161-177. [DOI:10.1016/B978-0-12-821571-5.00009-X]
6. Kırbaş Z, Kumcuoglu S, Tavman S. Effects of apple, orange and carrot pomace powders on gluten-free batter rheology and cake properties. Journal of food science and technology. 2019 Feb 5;56(2):914-26. [DOI:10.1007/s13197-018-03554-z]
7. Foschia M, Horstmann S, Arendt EK, Zannini E. Nutritional therapy-Facing the gap between coeliac disease and gluten-free food. International journal of food microbiology. 2016 Dec 19;239:113-24. [DOI:10.1016/j.ijfoodmicro.2016.06.014]
8. Das AB, Bhattacharya S. Characterization of the batter and gluten-free cake from extruded red rice flour. Lwt. 2019 Mar 1;102:197-204. [DOI:10.1016/j.lwt.2018.12.026]
9. Benkadri S, Salvador A, Zidoune MN, Sanz T. Gluten-free biscuits based on composite rice-chickpea flour and xanthan gum. Food Science and Technology International. 2018 Oct;24(7):607-16. [DOI:10.1177/1082013218779323]
10. Taetzsch A, Das SK, Brown C, Krauss A, Silver RE, Roberts SB. Are gluten-free diets more nutritious? An evaluation of self-selected and recommended gluten-free and gluten-containing dietary patterns. Nutrients. 2018 Dec 3;10(12):1881. [DOI:10.3390/nu10121881]
11. Ashraf S, Sofi SA, Muzaffar K, Majid D, Kour J. Nutritional Quality of Gluten-Free Breads. InGluten-free Bread Technology 2021 Oct 9 (pp. 89-99). Cham: Springer International Publishing. [DOI:10.1007/978-3-030-73898-3_6]
12. Kenny S, Wehrle K, Stanton C, Arendt EK. Incorporation of dairy ingredients into wheat bread: effects on dough rheology and bread quality. European Food Research and Technology. 2000 Apr;210(6):391-6. [DOI:10.1007/s002170050569]
13. Gallagher E, Gormley TR, Arendt EK. Recent advances in the formulation of gluten-free cereal-based products. Trends in Food Science & Technology. 2004 Mar 1;15(3-4):143-52. [DOI:10.1016/j.tifs.2003.09.012]
14. Macedo C, Nunes MC, Sousa I, Raymundo A. Rheology methods as a tool to study the impact of whey powder on the dough and breadmaking performance of wheat flour. Fluids. 2020 Apr 14;5(2):50. [DOI:10.3390/fluids5020050]
15. Meza BE, Verdini RA, Rubiolo AC. Viscoelastic behaviour of heat-treated whey protein concentrate suspensions. Food Hydrocolloids. 2009 May 1;23(3):661-6. [DOI:10.1016/j.foodhyd.2008.03.015]
16. Pico J, Reguilon MP, Bernal J, Gómez M. Effect of rice, pea, egg white and whey proteins on crust quality of rice flour-corn starch based gluten-free breads. Journal of Cereal Science. 2019 Mar 1;86:92-101. [DOI:10.1016/j.jcs.2019.01.014]
17. Crockett R, Ie P, Vodovotz Y. Effects of soy protein isolate and egg white solids on the physicochemical properties of gluten-free bread. Food chemistry. 2011 Nov 1;129(1):84-91. [DOI:10.1016/j.foodchem.2011.04.030]
18. Brnčić M, Bosiljkov T, Ukrainczyk M, Tripalo B, Brnčić SR, Karlović S, Karlović D, Ježek D, Topić DV. Influence of whey protein addition and feed moisture content on chosen physicochemical properties of directly expanded corn extrudates. Food and bioprocess technology. 2011 Oct;4(7):1296-306. [DOI:10.1007/s11947-009-0273-0]
19. Guinee TP, Caric M, Kalab M. Pasteurized processed cheese and imitation cheese products. In: Fox PF, editor. Cheese: Chemistry, Physics and Microbiology. Vol. 2. London: Elsevier; 2004. p. 349-394. [DOI:10.1016/S1874-558X(04)80052-6]
20. Ritz E, Hahn K, Ketteler M, Kuhlmann MK, Mann J. Phosphate additives in food-a health risk. Deutsches Ärzteblatt International. 2012 Jan 27;109(4):49. [DOI:10.3238/arztebl.2012.0049]
21. Shirashoji N, Aoyagi H, Jaeggi JJ, Lucey JA. Effect of tetrasodium pyrophosphate concentration and cooking time on the physicochemical properties of process cheese. Journal of Dairy Science. 2016 Sep 1;99(9):6983-94. [DOI:10.3168/jds.2016-10968]
22. Rubio-Aliaga I, Krapf R. Phosphate intake, hyperphosphatemia and kidney function. Pflügers Archiv-European Journal of Physiology. 2022 Aug;474(8):935-47. [DOI:10.1007/s00424-022-02691-x]
23. Panseri S, Arioli F, Biolatti C, Mosconi G, Pavlovic R, Chiesa LM. Detection of polyphosphates in seafood and its relevance toward food safety. Food Chemistry. 2020 Dec 1;332:127397. [DOI:10.1016/j.foodchem.2020.127397]
24. Skendi A, Papageorgiou M, Varzakas T. High protein substitutes for gluten in gluten-free bread. Foods. 2021 Aug 25;10(9):1997. [DOI:10.3390/foods10091997]
25. Moniri H, Farahmandfar R, Motamedzadegan A. Investigation of hot air and foam‐mat dried cress seed gum by FT‐IR, zeta potential, steady shear viscosity, dynamic oscillatory behavior and other physical properties. Food science & nutrition. 2020 Apr;8(4):2143-55. [DOI:10.1002/fsn3.1514]
26. Guimarães RR, Freitas MC, Silva VL. Simple cakes elaborated with flour of watermelon inner skin (Citrullus vulgaris, Sobral): chemical, physical and sensory evaluation. Food Science and Technology. 2010;30:354-63. [DOI:10.1590/S0101-20612010000200011]
27. Karp S, Wyrwisz J, Kurek MA, Wierzbicka A. Combined use of cocoa dietary fibre and steviol glycosides in low-calorie muffins production. International Journal of Food Science and Technology. 2017 Apr;52(4):944-53. [DOI:10.1111/ijfs.13358]
28. Huang W, Kim Y, Li X, Rayas-Duarte P. Rheofermentometer parameters and bread specific volume of frozen sweet dough influenced by ingredients and dough mixing temperature. Journal of Cereal Science. 2008 Nov 1;48(3):639-46. [DOI:10.1016/j.jcs.2008.02.008]
29. Komeroski MR, Homem RV, Schmidt HD, Rockett FC, de Lira L, da Farias DV, Kist TL, Doneda D, Rios AD, de Oliveira VR. Effect of whey protein and mixed flours on the quality parameters of gluten-free breads. International Journal of Gastronomy and Food Science. 2021 Jul 1;24:100361. [DOI:10.1016/j.ijgfs.2021.100361]
30. Ferreyra LS, Verdini RA, Soazo M, Piccirilli GN. Impact of whey protein addition on wheat bread fermented with a spontaneous sourdough. International Journal of Food Science and Technology. 2021 Sep;56(9):4738-45. [DOI:10.1111/ijfs.15052]
31. Drabińska N, Ciska E, Szmatowicz B, Krupa-Kozak U. Broccoli by-products improve the nutraceutical potential of gluten-free mini sponge cakes. Food chemistry. 2018 Nov 30;267:170-7. [DOI:10.1016/j.foodchem.2017.08.119]
32. Walstra P, Walstra P, Wouters JT, Geurts TJ. Dairy science and technology. CRC press; 2005 Sep 29. [DOI:10.1201/9781420028010]
33. Ziobro R, Witczak T, Juszczak L, Korus J. Supplementation of gluten-free bread with non-gluten proteins. Effect on dough rheological properties and bread characteristic. Food Hydrocolloids. 2013 Aug 1;32(2):213-20. [DOI:10.1016/j.foodhyd.2013.01.006]
34. Sahagún, M., & Gómez, M. (2018). Assessing influence of protein source on characteristics of gluten-free breads optimising their hydration level. Food and Bioprocess Technology, 11(9), 1686-1694. [DOI:10.1007/s11947-018-2135-0]
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Ramezani A, Farahmandfar R. Effects of Tetrasodium Pyrophosphate and Whey Protein Concentrate on Quantitative and Qualitative Characteristics of Gluten-free Breads. Nutr Food Health Dis 2026; 13 (2) :9-17
URL: http://nfsr.sbmu.ac.ir/article-1-675-en.html


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Volume 13, Issue 2 ( Apr-Jun 2026) Back to browse issues page
Nutrition and food in health and disease
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