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:: Volume 4, Issue 4 (Oct-Dec 2017) ::
Nutr Food Sci Res 2017, 4(4): 15-24 Back to browse issues page
Chemical Composition and Biological Activities of Lemon (Citrus limon) Leaf Essential Oil
Mohammad Hojjati * , Hassan Barzegar
Department of Food Science and Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran , hojjatim@yahoo.com
Abstract:   (11704 Views)

Background and Objectives: Essential oils and their compounds are getting increasing interest due to their multipurpose functionals as alternatives to the artificial preservatives. The aim of this study was to extract and identify the volatile constituents of essential oil of Citrus lemon leaf and to evaluate its antioxidant and antibacterial abilities.
Materials and Methods: The essential oil was extracted using Clevenger apparatus and characterized by means of gas chromatography mass spectrometry (GC-MS). Folin-Ciocalteu method was used to determine the total phenolic equivalent (TPC). The antioxidant properties of the essential oil were evaluated by 2,2- diphenyl-picrylhydrazyl (DPPH) assay and the detection of inhibitory effect of the essential oil at various concentrations (0.025-10 mg/mL) on Staphylococcus aureus, Bacillus cereus, Streptococcus faecium, Escherichia coli, Salmonella typhi and Shigella dysentery was carried out by agar disc-diffusion method and then Minimum Inhibitory Concentration (MIC) assay was evaluated.
Results: GC-MS analysis allowed the identification of twenty-seven compounds and linalool (30.62%), geraniol (15.91%), α-terpineol (14.52%), and linalyl acetate (13.76%) were the main constituents. TPC of essential oil was 14.73 mg gallic acid equivalent/g dry plant material and its IC50 value was found to be 0.98 mg/mL in DPPH test, so lemon leaf essential oil showed good free radical scavenging capacity at all studied concentrations. However the antioxidant capacity increased with increasing concentration of the essential oil. The results showed that increasing the essential oil concentration, increases the zone of inhibition, so that the highest antibacterial activity was observed at 10 mg/mL of essential oil.  The results of antimicrobial activity by disc diffusion method showed that essential oil exhibited maximum zone inhibition against Gram-positive bacteria including B. cereus, S. aureus and S. faecium, whereas the minimum zone inhibition was shown by S. typhi and S. dysentery as Gram-negative pathogens at the same concentrations. The highest MIC showed by S. aureus and S. dysentery, whereas the least MIC value was observed with B. cereus and S. typhi.
Conclusions: The results obtained in this study have shown the moderate biological potential of essential oil of C. limon leaf is probably due to its particular chemical composition, mainly the high amounts of linalool. Therefore, this essential oil could be used to raise the shelf life of foods as a natural preservative ingredient.
 
Keywords: Antibacterial, Antioxidant, Citrus, Essential oil, GC-MS
Full-Text [PDF 196 kb]   (26117 Downloads)    
Article type: Research | Subject: Food Science
Received: 2017/07/5 | Accepted: 2017/10/14 | Published: 2017/10/14
References
1. Tranchida PQ, Bonaccorsi I, Dugo P, Mondello L, Dugo G. Analysis of Citrus essential oils: state of the art and future perspectives. A review. Flavour and Fragrance Journal. 2012;27(2):98-123. [DOI:10.1002/ffj.2089]
2. Lota M-L, de Rocca Serra D, Tomi F, Casanova J. Chemical variability of peel and leaf essential oils of mandarins from Citrus reticulata Blanco. Biochemical Systematics and Ecology. 2000;28(1):61-78. [DOI:10.1016/S0305-1978(99)00036-8]
3. Vekiari SA, Protopapadakis EE, Papadopoulou P, Papanicolaou D, Panou C, Vamvakias M. Composition and seasonal variation of the essential oil from leaves and peel of a Cretan lemon variety. Journal of Agricultural and Food Chemistry. 2002;50(1):147-53. [DOI:10.1021/jf001369a] [PMID]
4. Golmakani MT, Moayyedi M. Comparison of heat and mass transfer of different microwave‐assisted extraction methods of essential oil from Citrus limon (Lisbon variety) peel. Food Science and Nutrition. 2015;3(6):506-18. [DOI:10.1002/fsn3.240] [PMID] [PMCID]
5. Ayedoun A, Sossou P, Mardarowicz M, Leclercq PA. Volatile constituents of the peel and leaf oils of Citrus limon L. Burm. f. from Benin. Journal of Essential Oil Research. 1996;8(4):441-4. [DOI:10.1080/10412905.1996.9700661]
6. Kirbaslar G, Kirbaslar SI. Composition of Turkish bitter orange and lemon leaf oils. Journal of Essential Oil Research. 2004;16(2):105-8. [DOI:10.1080/10412905.2004.9698663]
7. Lota M-L, de Rocca Serra D, Tomi F, Jacquemond C, Casanova J. Volatile components of peel and leaf oils of lemon and lime species. Journal of Agricultural and Food Chemistry. 2002;50(4):796-805. [DOI:10.1021/jf010924l]
8. Njoroge S, Ukeda H, Kusunose H, Sawamura M. Volatile components of Japanese yuzu and lemon oils. Flavour and Fragrance Journal. 1994;9(4):159-66. [DOI:10.1002/ffj.2730090404]
9. Djenane D. Chemical profile, antibacterial and antioxidant activity of Algerian citrus essential oils and their application in Sardina pilchardus. Foods. 2015;4(2):208-28. [DOI:10.3390/foods4020208] [PMID] [PMCID]
10. Jing L, Lei Z, Li L, Xie R, Xi W, Guan Y, et al. Antifungal activity of citrus essential oils. Journal of Agricultural and Food Chemistry. 2014;62(14):3011-33. [DOI:10.1021/jf5006148] [PMID]
11. Mondello L, Cotroneo A, Dugo G, Dugo P. Italian citrus petitgrain oils. Part IV. Composition of lemon petitgrain oil. Journal of Essential Oil Research. 1997;9(5):495-508. [DOI:10.1080/10412905.1997.9700766]
12. Ifesan B, Fashakin J, Ebosele F, Oyerinde A. Antioxidant and antimicrobial properties of selected plant leaves. European Journal of Medicinal Plants. 2013;3(3):465. [DOI:10.9734/EJMP/2013/3383]
13. Kivanç M, Akgül A. Antibacterial activities of essential oils from Turkish spices and citrus. Flavour and Fragrance Journal. 1986;1(4‐5):175-9. [DOI:10.1002/ffj.2730010409]
14. Huang Y, PU Z, Chen Q. The chemical composition of the leaf essential oils from 110 citrus species, cultivars, hybrids and varieties of Chinese origin. Perfumer and Flavorist. 2000;25(1):53-66.
15. Hamdan D, El-Readi, MZ, Tahrani A, Herrmann F, Kaufmann D, Farrag N, El-Shazly A, Wink M. The chemical constituents of Egyptian citrus species. Chemical composition and biological activity of Citrus jambhiri Lush. Food Chemistry. 2011;127(2): 394-403. [DOI:10.1016/j.foodchem.2010.12.129] [PMID]
16. Kamiyama S. Studies on leaf oils of Citrus species: Part I. Composition of leaf oils from Citrus unshiu, Citrus natsudaidai, Citrus kokitsu and Citrus limon. Agricultural and Biological Chemistry. 1967;31(9):1091-6.
17. Zandi-Sohani N, Ramezani L. Evaluation of five essential oils as botanical acaricides against the strawberry spider mite Tetranychus turkestani Ugarov and Nikolskii. International Biodeterioration and Biodegradation. 2015;98:101-6. [DOI:10.1016/j.ibiod.2014.12.007]
18. Singleton V, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965;16(3):144-58.
19. Raeisi M, Hashemi M, Aminzare M, Sadeghi M, Jahani T, Keshavarzi H, et al. Comparative Evaluation of phytochemical, antioxidant, and antibacterial properties from the essential oils of four commonly consuming plants in Iran. Journal of Food Quality and Hazards Control. 2016;3(3):107-13.
20. Hamdan D, El-Shazly A. The chemical constituents of Egyptian citrus species. An examination of the volatile oil and light petrolum fraction of rough lemon (Citrus jambahri Lush) leaf. European Scientific Journal. 2014;10(15):94-115.
21. Waikedre J, Dugay A, Barrachina I, Herrenknecht C, Cabalion P, Fournet A. Chemical composition and antimicrobial activity of the essential oils from new Caledonian Citrus macroptera and Citrus hystrix. Chemistry and biodiversity. 2010;7(4):871-7. [DOI:10.1002/cbdv.200900196] [PMID]
22. Khue DB, Tuan PM, Quan NV, Oanh DTB. Total phenolic content and anti-oxidant capacity of some spices and herbs in Vietnam. Journal of Post-Harvest Technology. 2013;1(1):22-8.
23. Agarwal M, Kumar A, Gupta R, Upadhyaya S. Extraction of polyphenol, flavonoid from Emblica officinalis, Citrus limon, Cucumis sativus and evaluation of their antioxidant activity. Oriental Journal of Chemistry. 2012;28(2):993-8. [DOI:10.13005/ojc/280248]
24. Papoutsis K, Vuong QV, Pristijono P, Golding JB, Bowyer MC, Scarlett CJ, Stathopoulos CE. Enhancing the total phenolic content and antioxidants of lemon pomace aqueous extracts by applying UV-C irradiation to the dried powder. Foods. 2016;5(3):55. [DOI:10.3390/foods5030055] [PMID] [PMCID]
25. Lagha-Benamrouche S, Madani K. Phenolic contents and antioxidant activity of orange varieties (Citrus sinensis L. and Citrus aurantium L.) cultivated in Algeria: peels and leaves. Industrial Crops and Products. 2013;50:723-30. [DOI:10.1016/j.indcrop.2013.07.048]
26. Majnooni M-B, Mansouri K, Gholivand MB, Mostafaie A, Mohammadi-Motlagh H-R, Afnanzade N-S, et al. Chemical composition, cytotoxicity and antioxidant activities of the essential oil from the leaves of Citrus aurantium L. African Journal of Biotechnology. 2012;11(2):498-503.
27. Hamdan D, Ashour ML, Mulyaningsih S, El-Shazly A, Wink M. Chemical composition of the essential oils of variegated pink-fleshed lemon (Citrus x limon L. Burm. f.) and their anti-inflammatory and antimicrobial activities. Zeitschrift für Naturforschung C. 2013;68(7-8):275-84. [DOI:10.5560/ZNC.2013.68c0275] [PMID]
28. Hamdan D, El-Readi M, Nibret E, Sporer F, Farrag N, El-Shazly A, Wink M. Chemical composition of the essential oils of two Citrus species and their biological activities. Die Pharmazie-An International Journal of Pharmaceutical Sciences. 2010;65(2):141-7.
29. El-hawary SS, Taha KF, Abdel-Monem AR, Kirillos FN, Mohamed AA. Chemical composition and biological activities of peels and leaves essential oils of four cultivars of Citrus deliciosa var. tangarina. American Journal of Essential Oils and Natural Products. 2013;1(2):1-6.
30. Lücker J, El Tamer MK, Schwab W, Verstappen FW, van der Plas LH, Bouwmeester HJ, et al. Monoterpene biosynthesis in lemon (Citrus limon). European Journal of Biochemistry. 2002;269(13):3160-71. [DOI:10.1046/j.1432-1033.2002.02985.x] [PMID]
31. AL-Jabri NN, Hossain MA. Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria. Beni-Suef University Journal of Basic and Applied Sciences. 2014;3(4):247-53. [DOI:10.1016/j.bjbas.2014.10.011]
32. FAOstat. Agriculture organization of the United Nations. Statistical database. 2014.
33. Tongnuanchan P, Benjakul S. Essential oils: extraction, bioactivities, and their uses for food preservation. Journal of Food Science. 2014;79(7):R1231-R49. [DOI:10.1111/1750-3841.12492] [PMID]
34. 5. Di Vaio C, Graziani G, Gaspari A, Scaglione G, Nocerino S, Ritieni A. Essential oils content and antioxidant properties of peel ethanol extract in 18 lemon cultivars. Scientia Horticulturae. 2010;126(1):50-5. [DOI:10.1016/j.scienta.2010.06.010]
35. Espina L, Somolinos M, Lorán S, Conchello P, García D, Pagán R. Chemical composition of commercial citrus fruit essential oils and evaluation of their antimicrobial activity acting alone or in combined processes. Food Control. 2011;22(6):896-902. [DOI:10.1016/j.foodcont.2010.11.021]
36. Azhdarzadeh F, Hojjati M. Chemical composition and antimicrobial activity of leaf, ripe and unripe peel of bitter orange (Citrus aurantium) essential oils. Nutrition and Food Sciences Research. 2016;3(1):43-50. [DOI:10.18869/acadpub.nfsr.3.1.43]
37. Srisukh V, Tribuddharat C, Nukoolkarn V, Bunyapraphatsara N, Chokephaibulkit K, Phoomniyom S, et al. Antibacterial activity of essential oils from Citrus hystrix (makrut lime) against respiratory tract pathogens. Science Asia. 2012;38:212-7. [DOI:10.2306/scienceasia1513-1874.2012.38.212]
38. Saeb S, Amin M, Gooybari RS, Aghel N. Evaluation of antibacterial activities of Citrus limon, Citrus reticulata, and Citrus grandis against pathogenic bacteria. International Journal of Enteric Pathogens. 2016;4(4):1-5. [DOI:10.15171/ijep.2016.13]
39. zengin H, Baysal AH. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules. 2014;19(11):17773-98. [DOI:10.3390/molecules191117773] [PMID]
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Hojjati M, Barzegar H. Chemical Composition and Biological Activities of Lemon (Citrus limon) Leaf Essential Oil. Nutr Food Sci Res 2017; 4 (4) :15-24
URL: http://nfsr.sbmu.ac.ir/article-1-244-en.html
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 4 (Oct-Dec 2017) Back to browse issues page
Nutrition and Food Sciences Research
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