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Cellulase production under solid state fermentation by ethanolic Zygomycetes fungi: Application of response surface methodology
Sanaz Behnam , Keikhosro Karimi , Morteza Khanahmadi
Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran
Abstract:   (62 Views)
Background and Objectives: Cellulase is an important enzyme with several applications in different industries, including food, laundry, pharmaceutical, textile, pulp and paper, and biofuels. Solid state fermentation is a method for cellulase production which has several advantages over submerged fermentation. In this paper, fungal cellulase was produced through solid state fermentation on wheat bran by three different filamentous fungi, i.e., Mucor indicus, Mucor hiemalis, and Rhizopus oryzae.
Materials and Methods: Effects of temperature, cultivation time, and moisture content of medium on cellulase production were investigated by applying response surface methodology. The experiments were performed using on an orthogonal central composite design. Based on analysis of variance, a quadratic model as a function of the three variables was suggested to express cellulase production. The optimum values of the parameters for cellulase production by the fungi were obtained, and the highest cellulase activity was reported.
Results and Conclusions: The fungi were able to produce appreciable amounts of cellulase. The models fitted to the experimental activities of the fungi had high regression coefficients. The optimum medium temperature for all fungi was 26.6 ºC. For M. indicus and R. oryzae, the optimum medium moisture content and cultivation time were 71.8% and 33.2 h, respectively, while they were 38.18% and 66.81 h for M. hiemalis. The highest cellulase activities by R. oryzae, M. indicus, and M. hiemalis were 281, 163, and 188 U per g of dry wheat bran, respectively, where the maximum enzyme production was achieved by R. oryzae. In conclusion, three advantageous strains can be successfully used for cellulase production through solid state fermentation with relatively high yields in comparison with other fungal strains.
Keywords: Cellulase, Mucor hiemalis, Mucor indicus, Rhizopus oryzae, Solid state fermentation
Type of Study: Research | Subject: General
Received: 2017/11/27 | Accepted: 2018/10/17
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Nutrition and Food Sciences Research
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