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:: Volume 5, Issue 1 (Jan-Mar 2018) ::
Nutr Food Sci Res 2018, 5(1): 41-46 Back to browse issues page
Evaluation of Chemical, Nutritional and Antioxidant Characteristics of Roselle (Hibiscus sabdariffa L.) Seed
Zohreh Mokhtari , Soheila Zarringhalami , Ali Ganjloo
Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, Iran , zaringhalami@znu.ac.ir
Abstract:   (6851 Views)
Background and Objectives: Roselle (Hibiscus sabdariffa L.) is one of the valuable plants grown in tropical and subtropical regions such as some parts of Iran. Roselle calyces are commonly used to make  herbal tea as well as a natural food color additive. However, Roselle seeds are usually discarded as by–products, while they can be used as a source of nutritious and functional compound. Therefore, in the current study, some of the chemical and nutritional characteristics of Iranian Roselle seed were investigated. In addition, the effects of different extracting solvents on seed extraction yield, total phenol content (TPC) and antioxidant activity (scavenging ability) of the extracts were evaluated.
Materials and Methods: Some chemical and nutritional properties of Roselle seeds such as moisture, protein, total fiber and lipid contents were determined. Also, fatty acid composition of the oil was determined using gas chromatography. The effects of different solvents including acetone, methanol and water, on yield, TPC (Folin–Ciocalteu assay) and antioxidant activity (DPPH and ABTS methods) of Roselle seeds were also investigated.
Results: The results showed that the Roselle seeds are the main sources of protein, lipid and fiber (26.62±0.03%, 21.03±0.02% and 19.81±0.01%, respectively). Linoleic (41.06±0.7%), oleic (27.07±0.01%) and palmitic (21.9±0.03%) acids are the main fatty acids in the Roselle seeds oil. The maximum yield (20±0.02%), related to aqueous extract, however, higher TPC (201±0.02 mg GAEs/100 g) and antioxidant activity (DPPH, 94.15±0.2%; ABTS, 75.91±0.03%) were observed in acetone extract.
Conclusions: According to the results, Roselle seeds are a good source of protein, fiber and oil, which contains unsaturated fatty acids especially linoleic acid. In addition, the acetone extract showed the highest TPC and antioxidant activity among other extracts.
 
Keywords: Antioxidant activity, Chemical and nutritional characteristics, Roselle seed, Total phenols
Full-Text [PDF 1239 kb]   (2477 Downloads)    
Article type: Research | Subject: Nutrition
Received: 2017/10/10 | Accepted: 2017/12/19 | Published: 2018/01/28
References
1. Tounkara F, Amadou I, Le GL, Shi, YH. Effect of boiling on the physicochemical properties of Roselle seeds (Hibiscus sabdariffa L.) cultivated in Mali. Afr J Biotechnol 2011; 10(79): 18160–18166.
2. Koehler HH, Chang IH, Scheier G, Burke W. Nutrition composition, protein quality and sensory properties of thirty–six cultivars of dry beans (P. vulgaris). J Food Sci 1987; 52: 1335–1340. [DOI:10.1111/j.1365-2621.1987.tb14076.x]
3. Parsa MB, Rezvani Moghaddam P, Ghorbani R, Azami Sardooei Z. The effect of sowing and plant density on yield and yield components of Roselle (Hibiscus sabdariffa L.) under Jiroft climate conditions. Ira J Field Crops Res 1395; 14(4): 735–745. [In Persian].
4. Nyam K, Leao SY, Tan CP, Long K. Functional properties of Roselle (Hibiscus sabdariffa L.) seed and its application as bakery product. J Food Sci Technol 2014; 51(12): 3830–3837. [DOI:10.1007/s13197-012-0902-x]
5. Shaheen MA, El–Nakhlawy F, Al–Shareef AR. Roselle (Hibiscus sabdariffa L.) seeds as unconventional nutritional source. Afr J Biotechnol 2012; 11(41): 9821–9824.
6. Elkhalifa AE, Mohamed MA, Agab M. Biochemical investigation on hibiscus sabdariffa l.(karkade) seed–based sudanese fermented food locally known as furundu. Electron. J Environ Agric Food Chem 2011; 11(03): 265–278.
7. Tran–Thi NY, Kasim NS, Yuliana M, Huynh LH, Chang WL, Ju YH. Polysaccharides–induced precipitation of protein from defatted Roselle seed and its characterization: Antinutritional factors and functional properties. J Taiwan Inst Chem Eng 2013; 44(2): 152–155. [DOI:10.1016/j.jtice.2012.09.010]
8. Bertagnolli SM, Silveira ML, Fogaça AD, Umann L, Penna NG. Bioactive compounds and acceptance of cookies made with Guava peel flour. J Food Sci Technol 2014; 34: 303–308. [DOI:10.1590/fst.2014.0046]
9. Rao N, Prabhakara PG, Rao G. Preparation of wood apple (Feronia limonia L.) seed protein concentrate and evaluation of its nutritional and functional characteristics. Int Food Res J 2011; 18(3): 949–955.
10. Oluwafemi R. Palm kernel cake (PKC) utilization in monogastic animal feeding implications for sustainable livestock development. J Vet Med 2009; 6(2): 1–8.
11. El–Adawy TA, Khalil AH. Characteristics of Roselle seeds as a new source of protein and lipid. J Agric Food Chem 1994; 42(9): 1896–1900. [DOI:10.1021/jf00045a013]
12. Mohamed R, Fern Andez M, Aguilar M. Roselle (Hibiscus sabdariffa) seed oil is a rich source of γ –tocopherol. J Food Sci 2007; 72(3): 207–211. [DOI:10.1111/j.1750-3841.2007.00285.x]
13. Eltayeib A, Elaziz A. Physicochemical properties of Roselle (Hibiscus sabdariffa L.) seeds oil 10– (Elrahad–1) in North Kordofan, Sudan. J Scientific Innov Res 2014; 3(6): 578–582.
14. Official Methods and Recommended Practices of the American Oil Chemists' Society, 5th ed., ed. D. Firestone, AOCS Press, Champaign, 1997.
15. Zarringhalami S, Sahari MA, Barzegar M, Hamidi–esfehani Z. Changes in oil content, chemical properties, fatty acid composition and triacylglycerol species of tea seed oil during maturity period. J Food Biochem 2011; 35: 1161–1169 [in Persian]. [DOI:10.1111/j.1745-4514.2010.00442.x]
16. Cissouma AI, Tounkara F, Nikoo M, Yang N, Xu X. Physicochemical properties and antioxidant activity of Roselle seed extracts. Adv J Food Sci Technol 2013; 5(11): 1483–1489. [DOI:10.19026/ajfst.5.3371]
17. Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 2009; 14: 2167–2180. [DOI:10.3390/molecules14062167]
18. Ahmed D, Khan MM, Saeed, R. Comparative analysis of phenolics, flavonoids, and antioxidant and antibacterial potential of methanolic, hexanic and aqueous extracts from adiantum caudatum leaves. Antioxidants 2015; 4: 394–409. [DOI:10.3390/antiox4020394]
19. Stalikas CD. Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 2007; 30: 326–895. [DOI:10.1002/jssc.200700261]
20. Do QD, Angkawijaya AE, Tran– Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Yi–Hsu J. Effect of extraction solvent on total phenol content, total flavonoids content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 2013; 22(3): 1–7.
21. Nzikou JM, Bouanga–Kalou G, Matos L, Ganongo–po FB, Mboungou–Mboussi PS, Moutoula FE, et al. Characteristics and nutritional evaluation of seeds oil from Roselle (Hibiscus sabdriffa L.) in Congo–Brazzaville. Int J Biol Sci 2011; 3: 141–146.
22. Halimatul SMN, Amin I, Mohd N, Nawalyah AG, Siti MM. Protein quality of Roselle (Hibiscus sabdariffa L.) seeds. Asean Food J 2007; 14 (2):131–140.
23. Anel TC, Thokchom R, Subapriya MS, Jenita Thokchom J, Singh SS. Hibiscus sabdariffa – A natural micro nutrient source. Inter J Adv Res Biol Sci 2016; 3(4): 243–248.
24. Mariod A. A, Suryaputra S, Hanafi M, Rohmana T, Leonardus B.S, Kardono L. B, Tjahjono Herwan T. Effect of diffrent processing technioues on Indonesian Roselle (Hibiscus radiates) seed constituents. Acta Sci Pol Technol 2013; 12(4): 359–364.
25. Hainida E, Amin I, Normah H, Mohd N. Nutritional and amino acid contents of differently treated Roselle (Hibiscus sabdariffa L.) seeds. J Food Chem 2008; 111: 906–911. [DOI:10.1016/j.foodchem.2008.04.070]
26. Savage GP. Chemical composition of walnuts (Juglans regia L.) grown in New Zealand. J Hum Nutr 2001; 56(1): 75–82.
27. Atta BM, Imaizumi K. Some characteristics of crude oil extracted from Roselle (Hibiscus sabdariffa L.) seeds cultivated in Egypt. J Oleo Sci 2002; 51(7): 457–461. [DOI:10.5650/jos.51.457]
28. Rehab FM, Ayman ME. Hypolipidemic and hypocholesterolemic effect of Roselle (Hibiscus sabdariffa L.) seeds oil in experimental Male rats. J Oleo Sci 2017; 66 (1):41–49. [DOI:10.5650/jos.ess16126]
29. Elneairy N. Comparative studies on Egyptian and Libyan Roselle seeds as a source of lipid and protein. Food Nutr Sci 2014; 5:2237–2245. [DOI:10.4236/fns.2014.522237]
30. Fernández–Agulló A, Pereira E, Freire MS, Valentao P, Andrade PB, González–Álvarez J, Pereira JA. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Ind Crops Prod 2013; 42: 126–132. [DOI:10.1016/j.indcrop.2012.05.021]
31. Prior R, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005; 53: 4290–4302. [DOI:10.1021/jf0502698]
32. Hashemi Z, Hojati M, Tahanezhad M. Evaluation of antioxidant activity of orange leaves (Citrus aurantium L.) compared with TBHQ synthetic antioxidant in edible oil. J Food Technol 1393; 2(6): 43–57. [In Persian].
33. Maizura M, Aminah A, Wan Aida WM. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiberofficinale) and turmeric (Curcuma longa) extract. Inter Food Res J 2011; 18: 529–534.
34. Tatiya AU, Tapadiya GG, Kotecha S, Surana SJ. Effect of solvents on total phenolics, antioxidant and antimicrobial properties of BrideliaretusaSprengstem bark. Indian J Nat Prod Resour 2011; 2 (4): 442–447.
35. Lapornik B, Prosek M, Wondra AG. Comparison of extracts prepared from plant byproducts using different solvents and extraction time. J Food Eng 2005; 71: 214–222. [DOI:10.1016/j.jfoodeng.2004.10.036]
36. Yilmaz Y, Toledo R. Oxygen radical absorbance capacities of grape/wine industry byproducts and effect of solvent type on extraction of grape seed polyphenols. J Food Compos Anal 2006; 19(1): 41–48. [DOI:10.1016/j.jfca.2004.10.009]
37. Rimamcwe KB, Chavan UD. Antioxidant activity and nutritional value of Roselle seeds flour. Int J Curr Microbiol Appl Sci 2017; 6(4): 2654–2663. [DOI:10.20546/ijcmas.2017.604.309]
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Mokhtari Z, Zarringhalami S, Ganjloo A. Evaluation of Chemical, Nutritional and Antioxidant Characteristics of Roselle (Hibiscus sabdariffa L.) Seed. Nutr Food Sci Res 2018; 5 (1) :41-46
URL: http://nfsr.sbmu.ac.ir/article-1-272-en.html


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Volume 5, Issue 1 (Jan-Mar 2018) Back to browse issues page
Nutrition and Food Sciences Research
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