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:: Volume 3, Issue 3 (Jul-Sep 2016) ::
Nutr Food Sci Res 2016, 3(3): 11-16 Back to browse issues page
Evaluation of Iron Bioavailability in Caco-2 cell Culture Model: Modification of the Original Method
Bahareh Nikooyeh, Tirang R. Neyestani *
Laboratory of Nutrition Research, National Nutrition and Food Technology Research Institute and Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran , neytr@yahoo.com
Abstract:   (5049 Views)

Background and Objectives: In the original in vitro method for evaluation of iron bioavailability using caco-2 cell model, ferritin formation by cells is considered as a functional indicator of both absorption and utilization of iron. However, as iron is measured in the tested material and then ferritin concentration is measured in caco-2 cell lysate, it is almost impossible to have a concrete measure of iron bioavailability because the percent of absorbable iron cannot be calculated by the concentration of iron in caco-2 cell lysate. To overcome this problem, the original method was modified by using ferrous sulfate tablet as a standard and also including blank well in the cell culture plate to subtract the baseline ferritin concentration from the ferritin concentration of other wells.

Materials and Methods: To evaluate iron bioavailability in this model, one kind of Iranian traditional breads (Sangak) was used. Standard curve was plotted using iron concentration and ferritin/protein ratio in the X and Y axes, respectively.

Results: The standard curve showed a linear equation: Ferritin (ng)/protein (mg) = (5.96 × bread Fe)+ 11.55, R2=0.989. Using this equation, the bioavailability of iron from the tested bread was 79.76 ± 11.5% of ferrous sulfate. Considering that the reported iron bioavailability from ferrous sulfate is about 10%, bioavailability of iron from the bread could be regarded 8%.

Conclusions: This data can be potentially usable to adjust the amount of absorbable iron in the target population for fortification programs.

Keywords: Iron, Bioavailability, Caco-2 cell

Keywords: Iron, Bioavailability, Caco-2 cell
Full-Text [PDF 356 kb]   (4827 Downloads)    
Article type: Research | Subject: Food Science
Received: 2015/12/27 | Accepted: 2016/07/18 | Published: 2016/07/18
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Nikooyeh B, Neyestani T R. Evaluation of Iron Bioavailability in Caco-2 cell Culture Model: Modification of the Original Method. Nutr Food Sci Res. 2016; 3 (3) :11-16
URL: http://nfsr.sbmu.ac.ir/article-1-145-en.html

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Volume 3, Issue 3 (Jul-Sep 2016) Back to browse issues page
Nutrition and Food Sciences Research
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