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:: Volume 5, Issue 3 (Jul-Sep 2018) ::
Nutr Food Sci Res 2018, 5(3): 43-48 Back to browse issues page
Effect of Cysteine and Lysine on Some Technical Properties of Yoghurt Starter Culture Bacteria
Nassim Azari , Hamid Ezzatpanah , Maryam Tajabadi-Ebrahimi, Maryam Mizani
Department of Food Science and Technology,Science and Research Branch,Islamic Azad University, Tehran,Iran
Abstract:   (2617 Views)
Background and Objectives: Enhancing the growth and function of lactic acid bacteria through fortifying the basic ingredient of yogurt, milk ,have always been a key factor in the application as an industrial starter culture. The purpose of the present study is to introduce the application of certain materials in yoghurt milk with the aim of enhancing the growth and acid production, and reduction of fermentation time, optimizing the structure and texture in fermented products as well as reducing starter culture consumption which results in balancing the corresponding expenditures.
Materials and Methods: By using local starter culture consisting Lactobacillus delbruecki ssp bulgaricus and Streptococcus thermophilus in 50:50 ratio, four samples of set yoghurt were prepared in triplicated tests.  One sample as a control and three samples were supplemented by L-lysine, L-cysteine and the mixture of the two by 100mg/L for each one respectively. The factors were studied based on fermentation time, bacterial viability, acid forming, sensory evaluation and the amount of syneresis  and water holding capacity (WHC).
Results: The results showed a significant effect on reducing fermentation time, this factor showed reduction from 260 minute in control sample to 210 minute in samples that were enriched by Lysine and Lysine plus cysteine and 180 minute in cysteine p≤0.05, significantly increasing titratable acidity and optimizing viability in enriched samples besides, the end product had firmer structure and a lower syneresis amounts as it was decreased from 4.71% in control sample to 2.83, 2.76 and 3.06% in samples that were enriched by lysine, cysteine and both of them respectively (p≤0.05) . the same results were found for the best WHC in cysteine fortification(p≤0.05).
Conclusions: Lysine has been responsible for optimization of Streptococcus thermophilus growth. Accordingly, there was an increase in the number of Streptococcus thermophilus to the advantage of Streptococcus thermophilus ratio which later led to a stronger start for fermentation process. On the other hand, as a result of cysteine reduction property enhanced condition for Lactobacillus bulgaricus led to a higher acid production, hence a stronger texture and lower wheying off and better firmness and thickness and finally better sensory evaluation score.
Keywords: Yoghurt milk, Starter culture, Syneresis, Water Holding Capacity
Full-Text [PDF 145 kb]   (900 Downloads)    
Protocol Study: Research | Subject: Food Science
Received: 2018/01/24 | Accepted: 2018/09/17 | Published: 2018/09/17
1. Mohammadi R, Sohrabvandi S, Mohammad Mortazavian A. The starter culture characteristics of probiotic microorganisms in fermented milks. Eng. Life Sci. 2012;12(4):399-409. [DOI:10.1002/elsc.201100125]
2. Michaylova M, Minkova S, Kimura K, Sasaki T, Isawa K. Isolation and characterization of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus from plants in Bulgaria. FEMS. microbiol. lett. 2007;269(1):160-9. [DOI:10.1111/j.1574-6968.2007.00631.x] [PMID]
3. Qin Y, Li J, Wang Q, Gao K, Zhu B, Lv N. [Identification of lactic acid bacteria in commercial yogurt and their antibiotic resistance]. Wei sheng wu xue bao= Acta microbiol. Sin. 2013;53(8):889-97.
4. Saccaro DM, Tamime AY, Pilleggi ALO, Oliveira MN. The viability of three probiotic organisms grown with yoghurt starter cultures during storage for 21 days at 4 C. Int J DairyTechnol.2009;62(3):397-404. [DOI:10.1111/j.1471-0307.2009.00497.x]
5. Robinson RK. Dairy microbiology handbook: the microbiology of milk and milk products: John Wiley & Sons; 2005.
6. Urshev Z, Ninova-Nikolova N, Ishlimova D, Pashova-Baltova K, Michaylova M, Savova T. Selection and characterization of naturally occurring high acidification rate Streptococcus thermophilus strains. Biotechnol Biotechnol Equip. 2014;28(5):899-903. [DOI:10.1080/13102818.2014.966233] [PMID] [PMCID]
7. Beshkova DM, Simova ED, Frengova GI, Simov ZI, Adilov EF. Production of amino acids by yogurt bacteria. Biotechnol prog. 1998;14(6):963-5. [DOI:10.1021/bp980082j] [PMID]
8. Sodini I, Lucas A, Oliveira M, Remeuf F, Corrieu G. Effect of milk base and starter culture on acidification, texture, and probiotic cell counts in fermented milk processing. J. Dairy Sci. 2002;85(10):2479-88. [DOI:10.3168/jds.S0022-0302(02)74330-0]
9. Colombo M, de Oliveira AEZ, de Carvalho AF, Nero LA. Development of an alternative culture medium for the selective enumeration of Lactobacillus casei in fermented milk. Food microbiol. 2014;39:89-95. [DOI:10.1016/j.fm.2013.11.008] [PMID]
10. Bong D, Moraru C. Use of micellar casein concentrate for Greek-style yogurt manufacturing: Effects on processing and product properties. J Dairy sci. 2014;97(3):1259-69. [DOI:10.3168/jds.2013-7488] [PMID]
11. Dave RI, Shah NP. Effect of cysteine on the viability of yoghurt and probiotic bacteria in yoghurts made with commercial starter cultures. Int Dairy J. 1997;7(8):537-45. [DOI:10.1016/S0958-6946(97)00053-8]
12. Dave RI. Factors affecting viability of yoghurt and probiotic bacteria in commercial starter cultures. Victoria University of Technology. 1998.
13. Tamime AY, Robinson RK. Yoghurt: science and technology: Woodhead Publishing; 1999.
14. Salvador A, Fiszman S. Textural and sensory characteristics of whole and skimmed flavored set-type yogurt during long storage. J. Dairy Sci. 2004;87(12):4033-41. [DOI:10.3168/jds.S0022-0302(04)73544-4]
15. La Torre L, Tamime A, Muir D. Rheology and sensory profiling of set‐type fermented milks made with different commercial probiotic and yoghurt starter cultures. Int J Dairy Tech. 2003;56(3):163-70. [DOI:10.1046/j.1471-0307.2003.00098.x]
16. Ott A, Hugi A, Baumgartner M, Chaintreau A. Sensory investigation of yogurt flavor perception: Mutual influence of volatiles and acidity. J Agric Food chem. 2000;48(2):441-50. [DOI:10.1021/jf990432x] [PMID]
17. Yilmaz M, Dertli E, Toker O, Tatlisu N, Sagdic O, Arici M. Effect of in situ exopolysaccharide production on physicochemical, rheological, sensory, and microstructural properties of the yogurt drink ayran: An optimization study based on fermentation kinetics. J Dairy sci. 2015;98(3):1604-24. [DOI:10.3168/jds.2014-8936] [PMID]
18. Kalab M, Allan-Wojtas P, Phipps-Todd BE. Development of microstructure in set-style nonfat yogurt-A review. J Food Struc. 1983;2(1):7.
19. Harwalkar V, Kalab M. Susceptibility of yoghurt to syneresis. Comparison of centrifugation and drainage methods. Milchwissenschaft. 1983.
20. Amatayakul T, Sherkat F, Shah NP. Syneresis in set yogurt as affected by EPS starter cultures and levels of solids. Int J Dairy Tech. 2006;59(3):216-21. [DOI:10.1111/j.1471-0307.2006.00264.x]
21. Akalın A, Unal G, Dinkci N, Hayaloglu A. Microstructural, textural, and sensory characteristics of probiotic yogurts fortified with sodium calcium caseinate or whey protein concentrate. J Dairy sci. 2012;95(7):3617-28. [DOI:10.3168/jds.2011-5297] [PMID]
22. Walstra P, Walstra P, Wouters JT, Geurts TJ. Dairy science and technology: CRC press; 2005.
23. Hansen EB. Commercial bacterial starter cultures for fermented foods of the future. Int J food microbiol. 2002;78(1):119-31. [DOI:10.1016/S0168-1605(02)00238-6]
24. Shakerian M, Hadi Razavi S, Khodaiyan F, Ziai SA, Saeid Yarmand M, Moayedi A. Effect of different levels of fat and inulin on the microbial growth and metabolites in probiotic yogurt containing nonviable bacteria. Int J Food Sci Tech. 2014;49(1):261-8. [DOI:10.1111/ijfs.12315]
25. Marshall VM, Rawson H. Effects of exopolysaccharide‐producing strains of thermophilic lactic acid bacteria on the texture of stirred yoghurt. Int J food sci tech. 1999;34(2):137-43. [DOI:10.1046/j.1365-2621.1999.00245.x]
26. Bhullar Y, Uddin M, Shah N. Effects of ingredients supplementation on textural characteristics and microstructure of yoghurt. Milchwissenschaft. 2002.
27. Remeuf F, Mohammed S, Sodini I, Tissier J. Preliminary observations on the effects of milk fortification and heating on microstructure and physical properties of stirred yogurt. Int Dairy J. 2003;13(9):773-82. [DOI:10.1016/S0958-6946(03)00092-X]
28. Puvanenthiran A, Williams R, Augustin M. Structure and visco-elastic properties of set yoghurt with altered casein to whey protein ratios. Int Dairy J. 2002;12(4):383-91. [DOI:10.1016/S0958-6946(02)00033-X]
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Azari N, Ezzatpanah H, Tajabadi-Ebrahimi M, Mizani M. Effect of Cysteine and Lysine on Some Technical Properties of Yoghurt Starter Culture Bacteria. Nutr Food Sci Res. 2018; 5 (3) :43-48
URL: http://nfsr.sbmu.ac.ir/article-1-288-en.html

Volume 5, Issue 3 (Jul-Sep 2018) Back to browse issues page
Nutrition and Food Sciences Research
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