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Effects of a Combination of Dietary Restriction and Exercise Training on Myocardial Apoptosis in Male Rats
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Hassan Pourrazi    , Afshar Jafari |
| Assistant Professor, Department of Physical Education, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran , purrazi.h@gmail.com |
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Abstract: (3732 Views) |
Background and Objectives: Apoptosis plays a key role in pathogenesis of various cardiovascular diseases due to loss of terminally differentiated cardiac myocytes. Therefore, the aim of this study was to investigate effects of combined dietary restriction and aerobic training on indices of myocardial apoptosis in male rats.
Materials and Methods: In this study, 32 three-month-old male rats were randomly divided into four major groups: 1) sedentary control (SED), 2)exercise training (EX), 3)diet restriction (DR) and 4)exercise training and diet restriction (EX+DR). All groups participated in the study for three months. Animals in SED and EX groups were fed ad libitum, while animals in DR and EX+DR groups were given access to 50% of the control animal diets. Animals in EX and EX+DR groups were involved in a three-month aerobic training program. Rat hearts were removed 48 h after the last training session. The Bax, Bcl-2 and caspase-3 gene expressions were analyzed using reverse-transcriptase polymerase chain reaction (RT-PCR). Data were analyzed using two-way ANOVA method.
Results: Results showed that the Bax gene expression and Bax/Bcl-2 ratio were significantly lower in trained rats than untrained rats (P<0.05). Furthermore, Bcl-2 gene expression was significantly higher in EX+DR group than other groups (P<0.05). However, exercise training and dietary restriction did not affect caspase-3 gene expression (P>0.05).
Conclusions: Overall, it seems that combination of dietary restriction and exercise training results in a significant increase in anti-apoptotic Bcl-2 gene expression. However, further studies are needed to show effects of combined dietary restriction and exercise training on apoptosis. |
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| Keywords: Bax, Bcl-2, Caspase-3, Dietary restriction, Exercise training |
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Full-Text [PDF 686 kb]
(1444 Downloads)
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Article type: Research |
Subject:
Nutrition
Received: 2018/11/29 | Accepted: 2019/02/14 | Published: 2019/08/4
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1. Roth GA, Johnson C, Abajobir A, Abd-Allah F, Abera SF, Abyu G, et al. Global, Regional, and National Burden of Cardiovascular Diseases for 10 Causes, 1990 to 2015. J Am Coll Cardiol. 2017; 70(1): 1-25. [ DOI:10.1016/j.jacc.2017.04.052] 2. Dadson K, Hauck L, Billia F. Molecular mechanisms in cardiomyopathy. Clinical Science. 2017; 131(13):1375-1392. [ DOI:10.1042/CS20160170] 3. Marzetti E, Wohlgemuth SE, Anton SD, Bernabei R, Carter CS, Leeuwenburgh C. Cellular mechanisms of cardioprotection by calorie restriction: state of the science and future perspectives. Clin Geriatr Med. 2009; 25(4): 715-732. [ DOI:10.1016/j.cger.2009.07.002] 4. Favaloro B, Allocati N, Graziano V, Ilio C.D, Laurenzi V.D. Role of Apoptosis in disease. Aging. 2012; 4(5):330-349. [ DOI:10.18632/aging.100459] 5. Lin YY, Hsieh PS, Cheng YJ, Cheng SM, Chen C, Huang CY, et al. Anti-apoptotic and Pro-survival Effects of Food Restriction on High-Fat Diet-Induced Obese Hearts. Cardiovasc Toxicol. 2017; 17:163-174. [ DOI:10.1007/s12012-016-9370-2] 6. Wencker D, Chandra M, Nguyen K, Miao W, Garantziotis S, Factor SM, et al. A mechanistic role for cardiac myocyte apoptosis in heart failure. J Clin Invest. 2003; 111:1497-1504. [ DOI:10.1172/JCI17664] 7. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. J Exerc Rehabil. 2013; 9(2):212-9. [ DOI:10.12965/jer.130002] 8. Ko IG, Kim SE, Kim CJ, Jee YS. Treadmill exercise alleviates aging-induced apoptosis in rat cardiac myocytes. Inter J Gerontology. 2013; 7(3):152-7. [ DOI:10.1016/j.ijge.2013.01.001] 9. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. J Appl Physiol. 2008; 105(6):1934-43. [ DOI:10.1152/japplphysiol.00037.2008] 10. Quindry JC, Miller L, McGinnis G, Kliszczewicz B, Irwin JM, Landram M, et al. Ischemia reperfusion injury, KATP channels, and exercise-induced cardioprotection against apoptosis. J Appl Physiol. 2012; 113(3):498-506. [ DOI:10.1152/japplphysiol.00957.2011] 11. Marin-Garcia J, Goldenthal MJ. Mitochondrial centrality in heart failure. Heart Fail Rev. 2008; 13(2):137-50. [ DOI:10.1007/s10741-007-9079-1] 12. Santana ET, Serra AJ, Silva Junior JA. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educacao Fisica. 2014; 20(2):233-238. [ DOI:10.1590/S1980-65742014000200015] 13. Walsh M, Shi Y, Remmen HV. The effects of dietary restriction on oxidative stress in rodents. Free Radic Biol Med. 2014; 66: 88-99. [ DOI:10.1016/j.freeradbiomed.2013.05.037] 14. Ma L, Wang R, Dong W, Li Y, Xu B, Zhang J. Long-term caloric restriction in mice may prevent age-related learning impairment via suppression of apoptosis. Behavioural Brain Research. 2016; 315: 45-50. [ DOI:10.1016/j.bbr.2016.07.036] 15. Patel BP, Safdar A, Raha S, Tarnopolsky MA, Hamadeh MJ. Caloric restriction shortens lifespan through an increase in lipid peroxidation, inflammation and apoptosis in the G93A mouse, an animal model of ALS. PLoS One. 2010; 5(2):e9386. [ DOI:10.1371/journal.pone.0009386] 16. Li X, Lu J, Wu W. Effect of Long-term Endurance Exercise on Cardiac Apoptosis. J of Mian Nor Univ. 2009; 7: 2009-11. 17. Natio H, Powers SK, Demirel HA and Aoki J. Exercise training increases heat shock protein in skeletal muscles of old rats. Med Sci Sports Exerc. 2001; 33(5): 729 -734. [ DOI:10.1097/00005768-200105000-00008] 18. Swift DL, Johannsen NM, Lavie CJ, Earnest CP, Church TS. The Role of Exercise and Physical Activity in Weight Loss and Maintenance. Prog Cardiovasc Dis. 2014; 56(4):441-447. [ DOI:10.1016/j.pcad.2013.09.012] 19. Fitzsimons DP, Bodell PW, Herrick RE, Baldwin KM. Left ventricular functional capacity in the endurance-trained rodent. J Appl Physiol. 1990; 69(1): 305-312. [ DOI:10.1152/jappl.1990.69.1.305] 20. Schaible TF, Scheuer J. Cardiac function in hypertrophied hearts from chronically exercised female rats. J Appl Physiol. 1981; 50(6): 1140-1145. [ DOI:10.1152/jappl.1981.50.6.1140] 21. Kemi OJ, Loennechen JP, Wisloff U, Ellingsen O. Intensity-controlled treadmill running in mice: cardiac and skeletal muscle hypertrophy. J Appl Physiol. 2002; 93(4): 1301-1309. [ DOI:10.1152/japplphysiol.00231.2002] 22. Allen DL, Harrison BC, Maass A, Bell ML, Byrnes WC, Leinwand LA. Cardiac and skeletal muscle adaptations to voluntary wheel running in the mouse. J Appl Physiol. 2001; 90(5):1900-1908. [ DOI:10.1152/jappl.2001.90.5.1900] 23. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and Bax gene expression in the rat heart. Gene Cell Tissue. 2015; 2(4): e32833. [ DOI:10.17795/gct-32833] 24. Fang J, Wu L, Chen L. Postconditioning attenuates cardiocyte ultrastructure injury and apoptosis by blocking mitochondrial permeability transition in rats. Acta Cardiol. 2008; 63(3): 377-387. [ DOI:10.2143/AC.63.3.1020316] 25. Vainshtein A, Kazak L, Hood DA. Effects of endurance training on apoptotic susceptibility in striated muscle. J Appl Physiol. 2011; 110(6):1638-45. [ DOI:10.1152/japplphysiol.00020.2011] 26. Arslan, S.; Erdem, S.; Sivri, A.; Hascelik, Z.; Tan, E. Exercise-induced apoptosis of rat skeletal muscle and the effect of meloxicam. Rheumatol Int. 2002; 21(4):133-136. [ DOI:10.1007/s00296-001-0156-9] 27. Liu WY, He W, Li H. Exhaustive training increases uncoupling protein 2 expression and decreases Bcl-2/Bax ratio in rat skeletal muscle. Oxid Med Cell Longev. 2013; 2013: 780719. [ DOI:10.1155/2013/780719] 28. Niemann B, Chen Y, Issa H, Silber RE, Rohrbach S. Caloric restriction delays cardiac ageing in rats: role of mitochondria. Cardio Rese. 2010; 88(2): 267-276. [ DOI:10.1093/cvr/cvq273] 29. Selman C, Gredilla R, Phaneuf Sh, Kendaiah S, Barja G, Leeuwenburgh Ch. Short-term caloric restriction and regulatory proteins of apoptosis in heart, skeletal muscle and kidney of Fischer 344 rats. Biogerontology. 2003; 4: 141-147. [ DOI:10.1023/A:1024149923693] 30. Siu, P. M.; Bryner, R. W.; Murlasits, Z.; Alway, S. E. Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle. J Appl Physiol. 2005; 99(1):204-209. [ DOI:10.1152/japplphysiol.00084.2005] 31. Kwak HB, Song W, Lawler JM. Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. FASEB. 2006; 20(6):791-3. [ DOI:10.1096/fj.05-5116fje] 32. Du J, Wang X, Miereles C, Bailey JL, Debigare R, Zheng B, et al. Activation of caspase-3 is an initial step triggering accelerated muscle proteolysis in catabolic conditions. J Clin Invest. 2004; 113:115-123. [ DOI:10.1172/JCI18330] 33. Ruetten H, Badorff C, Ihling C, Zeiher AM, Dimmeler S. Inhibition of caspase-3 improves contractile recovery of stunned myocardium, independent of apoptosis-inhibitory effects. J Am Coll Cardiol. 2001; 38: 2063-2070. [ DOI:10.1016/S0735-1097(01)01670-9] 34. Hu A, Jiao X, Gao E, Li Y, Sharifi-Azad S, Grunwald Z, et al. Tonic beta-adrenergic drive provokes proinflammatory and proapoptotic changes in aging mouse heart. Rejuvenation Res. 2008; 11: 215-226. [ DOI:10.1089/rej.2007.0609] |
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