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Showing 3 results for Essential Oils
Sepideh Khorasany, Mohamah Hosain Azizi, Mohsen Barzegar, Zohreh Hamidi Esfahani,
Volume 3, Issue 2 (4-2016)
Abstract
Background and Objectives: Essential oils (EOs) possess a wide range of significant properties including antiphlogistic, spasmolytic and antinociceptive effects. In this study, we use EOs from Thymus daenensis, Thymus caramanicus and Ziziphora clinopodiodes.
Materials and Methods: In this study, the plants were collected from the Botany Department, were air dried, grinded and set for extraction with a Clevenger-type apparatus, according to the procedure described in the European pharmacopoeia. In vitro antifungal activity of the EOs was evaluated according to Agar dilution method by determining the radial growth rate and inhibition ratio (%).
Results: Among the three EOs, T. daenensis contains the highest level of thymol (77.62%). Zizipora clinopodioides contains pulegone (31.21%), menth-3-en-8-0l (23.82%), menthol (7.21%), borneol (2.25%), carvacrol (5.38%) and piperitone (5.55%). Only the 9 µL concentration of Z. clinopodioides EO can prevent mycelium growth of both fungi for 7 days. Thymus caramanicus contains carvacrol (65.52%), p-cymene (13.21%), gamma-terpinene (4.44%), thymol (4.14%) and linalool (2.63).
Conclusions: According to the results, that compound thymol is more effective than carvacrol in preventing the growth of fungi.
Keywords: Essential oils, Aspergillus flavus, Aspergillus parasiticus, Natural antifungal
Razieh Partovi, Ali Khanjari, Sepideh Abbaszadeh, Aghil Sharifzadeh,
Volume 4, Issue 2 (4-2017)
Abstract
Background and Objectives: Vibrio parahaemolyticus is the causative agent of gastroenteritis due to consumption of contaminated seafood. The aim of the present study was to determine the chemical composition of Essential Oils (EOs) of five plants (Artemisia absinthium, Zataria multiflora Boiss., Pulicaria gnaphalodes, Trachyspermum ammi and Cuminum cyminum) and to evaluate their antimicrobial activity against pathogenic and non-pathogenic V. parahaemolyticus.
Materials and Methods: The EOs were analyzed by gas chromatography mass spectrometry. The detection of inhibitory effect of the EOs on the tested bacteria was carried out by agar disc-diffusion method and then MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of the EOs against both bacteria were determined.
Results: The analysis of the components of the EOs by gas chromatography mass spectrometry allowed the identification of 76 compounds. Of the five tested EOs, four oils exhibited an antimicrobial effect against both strains of V. parahaemolyticus. In all EOs tested, Pathogenic V. parahaemolyticus showed more sensitivity than non-pathogenic V. parahaemolyticus. The strongest EO against pathogenic V. parahaemolyticus were T. ammi with 63.42% thymol and C. cyminum with 29.02% cuminaldehyde and 20.70% α-terpinene-7-al, equally (31 mm inhibition zone). Non-pathogenic V. parahaemolyticus showed the most sensitivity against Z. multiflora Boiss. EO with 73.64% carvacrol (27 mm inhibition zone and the lowest MIC (0.025%) and MBC (0.05%)). Despite the large amount of thujone, A. absinthium EO in this study did not show antibacterial activity in disk diffusion assay, MIC or MBC values.
Conclusions: The results of this study suggest that Z. multiflora Boiss. and T. ammi have strong antimicrobial activity against both pathogenic and non-pathogenic V. parahaemolyticus.
Sajedeh Rezaiee, Fatemeh Ardestani, Morteza Khoshvaght,
Volume 8, Issue 3 (7-2021)
Abstract
Background and Objectives: In this study, antibacterial effects of whey protein coating with Mentha aquatica L. essential oil on Escherichia coli, Enterobacter and Klebsiella pneumoniae in Iranian white cheese were investigated.
Materials and Methods: Mentha aquatica L. essential oil was extracted using Clevenger apparatus. Mentha aquatica L. essential oil was added to each whey protein coating solution at 0.5, 1 and 1.5% concentrations. Whey protein treatment included cheese, bacteria and whey protein with no Mentha aquatica L. essential oil. Control treatment only included cheese and bacteria with no Mentha aquatica L. essential oil and whey protein. Minimal inhibitory concentration and minimal bactericidal concentration were assessed for the bacterial species. Antibacterial properties of the designed active coating system against Escherichia coli, Enterobacter and Klebsiella pneumoniae were assessed using direct cell counting and disk diffusion methods.
Results: Mentha aquatica L. essential oil at 1.5% concentration at the end of Day 15 of storage at 4 °C inhibited growth of Escherichia coli, Enterobacter and Klebsiella pneumoniae up to 100, 64 and 18%, respectively. To achieve the highest growth inhibition of Escherichia coli, Enterobacter and Klebsiella pneumoniae, 1.5, 1 and 1% of the essential oil were used to preserve organoleptic properties of the cheese.
Conclusions: Use of whey protein coating with 1.5% essential oil included no adverse effects on organoleptic, sensory and appearance characteristics of the cheese. Whey protein coating with Mentha aquatica L. essential oil inhibited growths of Escherichia coli completely and Enterobacter partially in Iranian white cheese.
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