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:: Volume 12, Issue 2 (Apr-Jun 2025) ::
Nutr Food Sci Res 2025, 12(2): 29-34 Back to browse issues page
Antibacterial Effects of Ethanolic Extractions of Aloe Vera, Black Tea, Pomegranate and Orange on Streptococcus mutans: An In-vitro Study
Bardia Vadiati Saberi , Soheil Taghavi Namin , Dina Maleki *
Dental Sciences Research Center, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran. , Dinamaleki74@gmail.com
Abstract:   (506 Views)
Background and Objectives: This study was designed to assess antibacterial effects of herbal alternatives such as aloe vera, black tea, pomegranate and orange extractions.
Materials and Methods: In this in-vitro study, extractions of Aloe barbadensis miller (aloe vera), Camellia sinensis assamica (black tea), Punica granatum (pomegranate) and Citrus spp. (orange) were prepared. The antibacterial activity of the extractions was assessed against Streptococcus mutans ATCC 35668 using well-diffusion technique. The microbial inhibition zones were assessed using antibiotic zone reader. The antibacterial activity was assessed using chromatography to report minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC). To analyze data, SPSS v.26.0 (IBM, Armonk, NY, USA) and ANOVA were used at the significance level of 0.05.
Results: All four extractions included a significant antibacterial effect against Streptococcus mutans. Pomegranate included the highest antibacterial effect, compared to black tea, aloe vera, orange and positive control. (P = 0.042, P = 0.025, P = 0.003 and P < 0.001, respectively) Following pomegranate, black tea included the highest antibacterial effect, compared to aloe vera, orange and positive control (P = 0.039, P = 0011 and P = 0.007, respectively). Aloe vera included a higher antibacterial effect than those orange and positive control did (P = 0.033 and P = 0.026, respectively). Orange included a higher antibacterial effect than that the positive control did (P = 0.048).
Conclusions: The studied herbal medicines were effective against one of the most important microorganisms of oral cavity, Streptococcus mutans, which is involved in tooth decay and gingival inflammation.
Keywords: Aloe, Black tea, Citrus, Herbal Medicine, Pomegranate, Streptococcus mutans
Full-Text [PDF 89 kb]   (242 Downloads)    
Article type: Research | Subject: Nutrition
Received: 2024/11/14 | Accepted: 2024/11/20 | Published: 2025/04/30

  • The primary goal of periodontal treatment is to stop the progression of the disease by decreasing the microbial biofilm formation, which contains a variety of bacteria.
  • The ethanolic extractions of pomegranate, black tea, aloe vera and orange included significant antibacterial effects against S. mutans.
  • Pomegranate included the strongest inhibition toward S. mutans followed by black tea, aloe vera and orange, respectively.
  • These herbal medicines, pomegranate, black tea, aloe vera and orange, can be considered as sufficient treatments in addition to conventional dental treatments, especially in the management of dental caries, gingivitis and periodontitis.
References
1. Zhou Y, Meyle J, Groeger S. Periodontal pathogens and cancer development. Periodontology 2000. 2024 Jul 4. [DOI:10.1111/prd.12590]
2. Dibello V, Custodero C, Cavalcanti R, Lafornara D, Dibello A, Lozupone M, Daniele A, Pilotto A, Panza F, Solfrizzi V. Impact of periodontal disease on cognitive disorders, dementia and depression: a systematic review and meta-analysis. GeroScience. 2024 Oct;46(5):5133-69. [DOI:10.1007/s11357-024-01243-8]
3. Bertolini M, Clark D. Periodontal disease as a model to study chronic inflammation in aging. Geroscience. 2024 Aug;46(4):3695-709. [DOI:10.1007/s11357-023-00835-0]
4. Wen P, Li H, Xu X, Zhang F, Zhao D, Yu R, Cheng T, Wang H, Yang C, Qin W, Yang X. A prospective study on maternal periodontal diseases and neonatal adverse outcomes. Acta Odontologica Scandinavica. 2024;83. [DOI:10.2340/aos.v83.40836]
5. Banihashem Rad SA, Esteves-Oliveira M, Maklennan A, Douglas GV, Castiglia P, Campus G. Oral health inequalities in immigrant populations worldwide: a scoping review of dental caries and periodontal disease prevalence. BMC public health. 2024 Jul 23;24(1):1968. [DOI:10.1186/s12889-024-19354-4]
6. Liu Z, Du X, Xu L, Shi Q, Tang X, Cao Y, Song K. The therapeutic perspective of cold atmospheric plasma in periodontal disease. Oral Diseases. 2024 Apr;30(3):938-48. [DOI:10.1111/odi.14547]
7. Rad FO, Mousavi E, Musapoor N, Maleki D, Khatibi N. Prevalence of C-Shaped Canals in Anterior and Posterior Teeth of Iranian Population Using Cone Beam Computed Tomography. Avicenna Journal of Dental Research. 2020 Jun 30;12(2):58-62. [DOI:10.34172/ajdr.2020.12]
8. Kinane DF, Lappin DF, Culshaw S. The role of acquired host immunity in periodontal diseases. Periodontology 2000. 2024 Apr 20. [DOI:10.1111/prd.12562]
9. Lima de Sousa T, Dourado D, Rodrigues JS, de Souza Rebouças J, Montes MA, Formiga FR. Treatment of periodontal disease: does drug delivery matter?. Frontiers in Bioengineering and Biotechnology. 2024 Jul 16;12:1427758. [DOI:10.3389/fbioe.2024.1427758]
10. Monteiro Viana JC, da Silva Gomes GE, Duarte Oliveira FJ, Marques de Araújo LN, Teles G, Mourão CF, de Vasconcelos Gurgel BC. The Role of Different Types of Cannabinoids in Periodontal Disease: An Integrative Review. Pharmaceutics. 2024 Jul 4;16(7):893. [DOI:10.3390/pharmaceutics16070893]
11. Javan R, Baghani Z, Foroumandi E. The effect of pomegranate versus chlorhexidine mouthwash or gel on periodontal disease: A systematic review and meta-analysis. Journal of Herbal Medicine. 2024 Oct 21:100955. [DOI:10.1016/j.hermed.2024.100955]
12. Graziani F, Izzetti R, Perić M, Marhl U, Nisi M, Gennai S. Early periodontal wound healing after chlorhexidine rinsing: a randomized clinical trial. Clinical Oral Investigations. 2024 Jun;28(6):1-0. [DOI:10.1007/s00784-024-05643-0]
13. Khanjani N, Masoomi F, Nikkhah M, Maleki A, Maleki D. Effective tooth brushing technique to manage periodontal diseases in orthodontic patients: a double-blind randomized controlled trial. Journal of Dentomaxillofacial Radiology, Pathology and Surgery. 2021 Oct 10;10(4):12-6.
14. Kuo TY, Hsieh MC, Cheng CD, Huang RY, Van Dyke TE, Sung CE, Wang CY, Hsieh YS, Cheng WC. Chlorhexidine gel topical application ameliorates inflammatory bone loss in experimental periodontitis. Journal of the Formosan Medical Association. 2023 Sep 1;122(9):899-910. [DOI:10.1016/j.jfma.2023.02.001]
15. Hussain U, Alam S, Rehman K, Antonoglou GN, Papageorgiou SN. Effects of chlorhexidine use on periodontal health during fixed appliance orthodontic treatment: a systematic review and meta-analysis. European Journal of Orthodontics. 2023 Feb 1;45(1):103-14. [DOI:10.1093/ejo/cjac044]
16. Ayoob A, Janakiram C, Priya MK. Spice-Based Herbal Oral Care Products as an Intervention in the Periodontal Diseases: A Systematic Scoping Review. Journal of Herbal Medicine. 2024 Jun 1;45:100862. [DOI:10.1016/j.hermed.2024.100862]
17. Mosaddad SA, Hussain A, Tebyaniyan H. Green alternatives as antimicrobial agents in mitigating periodontal diseases: a narrative review. Microorganisms. 2023 May 11;11(5):1269. [DOI:10.3390/microorganisms11051269]
18. Shaheen MY, Al-Zawawi AS, Divakar DD, Aldulaijan HA, Basudan AM. Role of chlorhexidine and herbal oral rinses in managing periodontitis. International dental journal. 2023 Apr 1;73(2):235-42. [DOI:10.1016/j.identj.2022.06.027]
19. ABU-SEIDA AM, Heba SE. Aloe vera in dentistry: current status and future prospects. International Arab Journal of Dentistry (IAJD). 2023 Nov 15;14(2):188-99. [DOI:10.70174/iajd.v14i2.943]
20. Andhare MG, Shetty S, Vivekanandan G, Shetty RM, Rahman B, Shetty SR, Siddeshappa ST, Desai V. Clinical efficacy of green tea, aloe vera and chlorhexidine mouthwashes in the treatment of dental biofilm induced gingivitis: A multi‐arm, double‐blinded, randomized controlled clinical trial. International Journal of Dental Hygiene. 2024 Aug;22(3):504-13. [DOI:10.1111/idh.12664]
21. Sharma R. Three Plants Kings of Dentistry: A Review on Aloe Vera, Allium Sativum and Mangifera Indica. Journal of Mahatma Gandhi University of Medical Sciences and Technology. 2024 Apr 20;8(1):20-5. [DOI:10.5005/jp-journals-10057-0224]
22. Tawfic HM, Niazy MA, Elsharkawy DA. Evaluation of the Antibacterial Effect of Aloe Vera versus Diode Laser in Management of Deep Carious Lesions (In Vivo Study). Al-Azhar Journal of Dentistry. 2023;10(3):9. [DOI:10.58675/2974-4164.1564]
23. Ehsani H, Mehrani J, Jabbareh L, Habibi E, Mousazadeh M, Maleki D, Tayyebe Khabbaz S. Comparison of Oral Chlorhexidine with Green Tea Mouthwash in Treatment of Patients with Chronic Generalized Periodontitis: A Double-Blind Controlled Randomized Clinical Trial. Journal of Dentomax-illofacial Radiology, Pathology and Surgery. 2019 Jun 10;8(2):7-12.
24. Çakır A, Şahin TN. Evaluation of the impact of fluoride in drinking water and tea on the enamel of deciduous and permanent teeth. BMC oral health. 2023 Aug 13;23(1):565. [DOI:10.1186/s12903-023-03267-6]
25. Marrone G, Basilicata M, Di Lauro M, Vita C, Masci C, Klinger FG, Cornali K, Maddaloni G, Bollero P, De Lorenzo A, Noce A. Healthy Effects of Pomegranate (Punica granatum L.) in Internal Medicine and Dentistry. Applied Sciences. 2024 Feb 16;14(4):1570. [DOI:10.3390/app14041570]
26. Karabulut H, Ulag S, Dalbayrak B, Arisan ED, Taskin T, Guncu MM, Aksu B, Valanezhad A, Gunduz O. A novel approach for the fabrication of 3D-printed dental membrane scaffolds including antimicrobial pomegranate extract. Pharmaceutics. 2023 Feb 22;15(3):737. [DOI:10.3390/pharmaceutics15030737]
27. Vieira AP, Danelon M, Fernandes GL, Berretta AA, Buszinski AF, Dos Santos L, Delbem AC, Barbosa DB. Pomegranate extract in polyphosphate-fluoride mouthwash reduces enamel demineralization. Clinical Oral Investigations. 2024 Jan 26;28(1):119. [DOI:10.1007/s00784-024-05495-8]
28. Gallas JA, Pelozo LL, Corona SA, Shen Y, Haapasalo M, Sousa‐Neto MD, Souza‐Gabriel AE. Effect of pomegranate solution alone or combined with chlorhexidine against oral multispecies biofilm. International Endodontic Journal. 2024. [DOI:10.1111/iej.14135]
29. Vinícius CT, Mancioppi CN, Adornes GJ, Pereira OW, Milori CS, Evangelista SG. Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging. Restorative Dentistry and Endodontics. 2024;49(1):1-4. [DOI:10.5395/rde.2024.49.e9]
30. Mulyanti S, Julaeha E, Rukayadi Y, Amaliya A. Anti-Plaque Potential of Lime (Citrus Aurantifolia) Peel Essential Oil: A Scoping Review.
31. Torshabi M, MoadabShoar Z, Negahban M. Preparation of Citrus reticulata peel nano‐encapsulated essential oil and in vitro assessment of its biological properties. European Journal of Oral Sciences. 2023 Apr;131(2):e12924. [DOI:10.1111/eos.12924]
32. Sugiaman VK, Djuanda R, Naliani S, Alfiyola E, Winardi J, Demolsky WL. Antibacterial Differences Effect between the Onion Extract (Allium cepa L.) and Lemon Juice (Citrus limon (L.) Burm. f.) on In Vitro Growth of Enterococcus faecalis. Journal of International Dental and Medical Research. 2023;16(1):111-6.
33. Setiawan AS, Subarnas A, Djais A, Milanda T, Ichwan S. Antibacterial Effect of Green Tea Leaves and Pomegranate Arils Ethanol Extract towards Streptococcus mutans ATCC 25175 and Veillonella parvula ATCC 10790T. Malaysian Journal of Medicine & Health Sciences. 2024 Jun 3;20.
34. Asmin P, Nusrath F. The Effects of Fresh Fruit Extracts and Commercially Available Fruit Extracts on the Growth of Streptococcus Mutans-An In Vitro Study [Internet]. 2023
35. Al-Qaralusi DM, Al-Mizraqchi AS. Effect of Non-Nutritive Sweeteners on Antibacterial Activity of Black and Green Tea Aqueous Extracts against Salivary Mutans Streptococci (in-vitro Study). Archives of Razi Institute. 2023 Feb 1;78(1):485-92.
36. Shakerian M, Khorasani MM, Doostaki S. In vitro effect of hydroalcoholic extract of aloe vera and 0.2% chlorhexidine mouthwash on streptococcus sanguinis, streptococcus salivarius and streptococcus mutans. Journal of Dental School. 2018 Jan 1;36(1):18-22.
37. Katuromunda M, Ssekatawa K, Niwagaba S. Evaluation of the antibacterial activity of lemon fruit juice, mondia whitei ethanolic extract and their combination against Streptococcus mutans.
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Vadiati Saberi B, Taghavi Namin S, Maleki D. Antibacterial Effects of Ethanolic Extractions of Aloe Vera, Black Tea, Pomegranate and Orange on Streptococcus mutans: An In-vitro Study. Nutr Food Sci Res 2025; 12 (2) :29-34
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 2 (Apr-Jun 2025) Back to browse issues page
Nutrition and food in health and disease
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