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Molecular Identification of Lactobacillus acidophilus Strain ABC240 Isolated from Traditional Yogurt and Assessing Its Stability and Safety as well as Antibacterial and Antioxidant Activities
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چکیده: (176 مشاهده) |
Background and Objectives: In recent years, research has focused on the isolation of new bacterial strains with diverse and beneficially biological activities. This study investigated stability (under various acid concentrations, various bile-salt concentrations, cholesterol absorption ability and surface hydrophobicity) and antagonistic, antioxidant and immune activities of Lactobacillus acidophilus strain ABC240 isolated from traditional yogurts.
Materials and Methods: Strain was identified using polymerase chain reaction technique. Its viability was assessed under acidic conditions (pH values of 3, 4 and 5) and in presence of bile salts (concentrations of 0.3, 0.5 and 0.7%). Additionally, antimicrobial activity was assessed through well diffusion and disc diffusion methods. Antioxidant capacity was assessed using ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays. This study analyzed the microbial cell surface hydrophobicity, cholesterol absorption ability and safety parameters, including antibiotic sensitivity, hemolytic activity, DNase activity and biogenic amine production.
Results: Results demonstrated high stability of this strain against various concentrations of acid (pH values of 3, 4 and 5) and bile salts (0.3, 0.5 and 0.7%). Ability of Lactobacillus acidophilus strain ABC240 to absorb cholesterol included 43.60% ±0.66 and its cell surface hydrophobicity was assessed at 50.49% ±0.63. The antimicrobial potential assessments revealed strong activity against several bacterial species. Furthermore, antioxidant activities, assessed using DPPH and ABTS methods, were detected as 44.69% ±0.89 and 49.80% ±0.79, respectively, indicating the bacterial high potential in inhibiting free radicals. Antibiotic sensitivity assay showed a non-growth zone diameter ranging from 13.20 mm ±0.55 (vancomycin) to 24.50 mm ±0.66 (chloramphenicol). Lactobacillus acidophilus strain ABC240 showed no hemolytic or DNase activity and did not produce biogenic amines.
Conclusions: In conclusion, Lactobacillus acidophilus strain ABC240 can be introduced as a new probiotic strain with biological and functional characteristics appropriate for use in the food industry. |
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متن کامل [PDF 790 kb]
(100 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
Food Science
دریافت: 1403/5/29 | پذیرش: 1403/7/6 | انتشار: 1403/9/3
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