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جلد 8 شماره 4 صفحات 36-29 برگشت به فهرست نسخه ها
Screening of Melamine in Milk and Milk-based Products Using Enzyme-linked Immunosorbent Assay
چکیده:   (656 مشاهده)
Background and Objectives: Melamine is an organic nitrogen used as an adulterant to increase nitrogen and protein contents in milks. Enzyme-linked immunosorbent assay has verified as a good alternative for rapid screening of melamine and other food additives instead of the laborious chromatography techniques.
Materials and Methods: In the present study, MaxSignal enzyme-linked immunosorbent assay  kit was used to screen melamine in 30 samples, including milks, infant formulas, chocolates, tea whiteners and pet foods. The kit was based on a competitive colorimetric enzyme-linked immunosorbent assay.
Results: Melamine was detected in the range of 0.05–0.69 ppm in infant formula/milk powder samples and 0.001–0.042 ppm in liquid milk samples, which were under the set limits of Codex Alimentarius, 2012. Codex Alimentarius Commission adopted a maximum melamine level of 1 ppm for powdered infant formulas and of 2.5 ppm for other foods and animal foods. The commission has now set a maximum limit of 0.15 ppm for melamine in liquid milks. Further protein analyses of milk and infant formula samples were also carried out to verify the presence of melamine.
Conclusions: It has been seen that enzyme-linked immunosorbent assay could  effectively be used as a screening tool for detecting melamine in a wide range of food samples. Whereas, complementary techniques such as high-performance liquid chromatography, gas chromatography/mass spectrometry can be adopted as verification methods for regulatory compliance.
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نوع مطالعه: پژوهشي | موضوع مقاله: صنایع غذایی
دریافت: 1399/12/5 | پذیرش: 1400/6/13 | انتشار: 1400/7/25
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Saeed S, Rashid A A, Rizvi Y A, Shehzad K, Nawaz S, Muhammad Y. Screening of Melamine in Milk and Milk-based Products Using Enzyme-linked Immunosorbent Assay. Nutr Food Sci Res. 2021; 8 (4) :29-36
URL: http://nfsr.sbmu.ac.ir/article-1-490-fa.html

Screening of Melamine in Milk and Milk-based Products Using Enzyme-linked Immunosorbent Assay. 1. 1400; 8 (4) :36-29

URL: http://nfsr.sbmu.ac.ir/article-1-490-fa.html



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دوره 8، شماره 4 - ( 7-1400 ) برگشت به فهرست نسخه ها
Nutrition and Food Sciences Research
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