en Nutrition Nutrition پژوهشي Research <p><strong>Background and Objectives: </strong>Essential oils (EOs) possess a wide range of significant properties including antiphlogistic, spasmolytic and antinociceptive effects. In this study, we use EOs from <em>Thymus daenensis, Thymus caramanicus and Ziziphora clinopodiodes.</em></p> <p><strong>Materials and Methods: </strong>In this study, the plants were collected from the Botany Department, were air dried, grinded and set for extraction with a Clevenger-type apparatus, according to the procedure described in the European pharmacopoeia. <em>In vitro</em> antifungal activity of the EOs was evaluated according to Agar dilution method by determining the radial growth rate and inhibition ratio (%).</p> <p><strong>Results: </strong>Among the three EOs, <em>T. daenensis </em>contains the highest level of thymol<em> (77.62%). Zizipora clinopodioides </em>contains pulegone (31.21%), menth-3-en-8-0l (23.82%), menthol (7.21%), borneol (2.25%), carvacrol (5.38%) and piperitone (5.55%). Only the 9 &micro;L concentration of <em>Z. clinopodioides </em>EO can prevent mycelium growth of both fungi for 7 days.<em> Thymus caramanicus </em>contains carvacrol (65.52%), p-cymene (13.21%), gamma-terpinene (4.44%), thymol (4.14%) and linalool (2.63).</p> <p><strong>Conclusions: </strong>According to the results, that compound thymol is more effective than carvacrol in preventing&nbsp;&nbsp; the growth of fungi.</p> <p><strong>Keywords: </strong>Essential oils,<em> Aspergillus flavus, Aspergillus parasiticus, </em>Natural antifungal</p> Essential oils, Aspergillus flavus, Aspergillus parasiticus, Natural antifungal 35 42 http://nfsr.sbmu.ac.ir/browse.php?a_code=A-10-454-1&slc_lang=en&sid=1 Sepideh Khorasany khorasany_mak@yahoo.com 10031947532846001818 10031947532846001818 No Tarbiat Modares of university Mohamah Hosain Azizi azizit_m@modares.ac.ir 10031947532846001819 10031947532846001819 Yes Tarbiat Modares of university Mohsen Barzegar 10031947532846001820 10031947532846001820 No Tarbiat Modares of university Zohreh Hamidi Esfahani 10031947532846001821 10031947532846001821 No Tarbiat Modares of university