:: Volume 6, Issue 4 (Oct-Dec 2019) ::
Nutr Food Sci Res 2019, 6(4): 9-16 Back to browse issues page
Effects of Eight Weeks of Resistance Training on Muscle Myostatin Gene Expression and Insulin Resistance in Male Wistar Rats with Type 2 Diabetes
Majid Hassan Qomi , Sajad Arshadi , Abdolali Banayifar , Yaser Kazemzadeh
Assistant Professor, Department of Sport Physiology, Islamic Azad University, South Tehran Branch, Tehran, Iran
Abstract:   (3417 Views)
Background and Objectives: Muscular atrophy is one of the indicators of uncontrolled diabetes. The aim of the current study was to investigate effects of eight weeks of resistance training (RT) on myostatin gene expression in soleus muscles and insulin resistance in streptozotocin (STZ)-induced diabetic rats.
Materials and Methods: In general, 14 Wistar male rats weighing 200–250 g and aging 8–10 weeks were selected for the study. Then, a newly prepared diabetic STZ solution was intraperitoneally injected to the rats. Animals were randomly divided into two groups of controls and resistance training diabetes. The resistance training protocol was carried out at ten repetitions as climbing up the ladder with 100% of the body weight for eight weeks, five days a week. Nearly 48 h after the last training session, soleus muscles of the rats were removed and the myostatin gene expression was assessed. Statistical data analysis was carried out using independent t-test at a significance level of P <0.05.
Results: Results of the independent t-test showed that the mean expression rate of myostatin protein genes in rat soleus muscles of the resistance training group was significantly lower than that of the control group (P = 0.013). Furthermore, levels of glucose, insulin and insulin resistance were significantly lower in resistance training group, compared to those in control group (P-values of 0.001, 0.005 and 0.001, respectively). A significantly positive correlation was seen between the mean expression of myostatin protein gene and the blood glucose (P = 0.012, r = 0.539), as well as the expression of myostatin protein gene and the insulin resistance (P = 0.015, r = 0.525).
Conclusions: Results of this study indicated that resistance training decreased myostatin expression and could improve insulin resistance in rats with type 2 diabetes. Hence, targeting myostatin might be a new therapeutic approach for the treatment of metabolic diseases such as obesity, diabetes and metabolic syndrome.
Keywords: Type 2 diabetes, Insulin resistance, Myostatin gene expression, Soleus muscle, Resistance training
Full-Text [PDF 581 kb]   (1435 Downloads)    
Article type: Research | Subject: Food Science
Received: 2019/06/3 | Accepted: 2019/06/3 | Published: 2019/06/3
References
1. Islam MS, du Loots TJM, experimental fi, pharmacology c. Experimental rodent models of type 2 diabetes: a review. 2009;31(4):249-61. [DOI:10.1358/mf.2009.31.4.1373958]
2. Riddell M, Iscoe KJPd. Physical activity, sport, and pediatric diabetes. 2006;7(1):60-70. [DOI:10.1111/j.1399-543X.2006.00146.x]
3. Perry BD, Caldow MK, Brennan-Speranza TC, Sbaraglia M, Jerums G, Garnham A, et al. Muscle atrophy in patients with Type 2 Diabetes Mellitus: roles of inflammatory pathways, physical activity and exercise. 2016;22:94.
4. Andersen H, Gadeberg P, Brock B, Jakobsen JJD. Muscular atrophy in diabetic neuropathy: a stereological magnetic resonance imaging study. 1997;40(9):1062-9. [DOI:10.1007/s001250050788]
5. Hulmi JJ, Silvennoinen M, Lehti M, Kivelä R, Kainulainen HJAJoP-H, Physiology C. Altered REDD1, myostatin and Akt/mTOR/FoxO/MAPK signaling in streptozotocin-induced diabetic muscle atrophy. 2011. [DOI:10.1152/ajpendo.00398.2011]
6. McPherron AC, Lawler AM, Lee S-JJN. Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member. 1997;387(6628):83. [DOI:10.1038/387083a0]
7. Wehling M, Cai B, Tidball JGJTFJ. Modulation of myostatin expression during modified muscle use. 2000;14(1):103-10. [DOI:10.1096/fasebj.14.1.103]
8. McCroskery S, Thomas M, Maxwell L, Sharma M, Kambadur RJTJocb. Myostatin negatively regulates satellite cell activation and self-renewal. 2003;162(6):1135-47. [DOI:10.1083/jcb.200207056]
9. Rios R, Carneiro I, Arce VM, Devesa JJAJoP-CP. Myostatin is an inhibitor of myogenic differentiation. 2002. [DOI:10.1152/ajpcell.00372.2001]
10. Jespersen J, Nedergaard A, Andersen L, Schjerling P, Andersen JJSjom, sports si. Myostatin expression during human muscle hypertrophy and subsequent atrophy: increased myostatin with detraining. 2011;21(2):215-23. [DOI:10.1111/j.1600-0838.2009.01044.x]
11. Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JAJD. Increased secretion and expression of myostatin in skeletal muscle from extremely obese women. 2009;58(1):30-8. [DOI:10.2337/db08-0943]
12. Hittel DS, Axelson M, Sarna N, Shearer J, Huffman KM, Kraus WEJM, et al. Myostatin decreases with aerobic exercise and associates with insulin resistance. 2010;42(11):2023. [DOI:10.1249/MSS.0b013e3181e0b9a8]
13. Gonzalez-Cadavid NF, Bhasin SJCOiCN, Care M. Role of myostatin in metabolism. 2004;7(4):451-7. [DOI:10.1097/01.mco.0000134365.99523.7f]
14. Milan G, Dalla Nora E, Pilon C, Pagano C, Granzotto M, Manco M, et al. Changes in muscle myostatin expression in obese subjects after weight loss. 2004;89(6):2724-7. [DOI:10.1210/jc.2003-032047]
15. Wilkes JJ, Lloyd DJ, Gekakis NJD. Loss-of-function mutation in myostatin reduces tumor necrosis factor α production and protects liver against obesity-induced insulin resistance. 2009;58(5):1133-43. [DOI:10.2337/db08-0245]
16. Isaacs A, Critchley J, Tai SS, Buckingham K, Westley D, Harridge S, et al. Exercise Evaluation Randomised Trial (EXERT): a randomised trial comparing GP referral for leisure centre-based exercise, community-based walking and advice only. 2007;11(10):1-165, iii-iv. [DOI:10.3310/hta11100]
17. Gordon B, Benson A, Bird S, Fraser SJDr, practice c. Resistance training improves metabolic health in type 2 diabetes: a systematic review. 2009;83(2):157-75. [DOI:10.1016/j.diabres.2008.11.024]
18. Lopez A, Wyatt F, editors. The Effect of Aerobic and Resistance Training on Glycemic Control in Type 2 Diabetes Mellitus: Meta-Analytic Study. International Journal of Exercise Science: Conference Proceedings; 2019.
19. Ahtiainen JP. Physiological and Molecular Adaptations to Strength Training. Concurrent Aerobic and Strength Training: Springer; 2019. p. 51-73. [DOI:10.1007/978-3-319-75547-2_5]
20. Matsakas A, Bozzo C, Cacciani N, Caliaro F, Reggiani C, Mascarello F, et al. Effect of swimming on myostatin expression in white and red gastrocnemius muscle and in cardiac muscle of rats. 2006;91(6):983-94. [DOI:10.1113/expphysiol.2006.033571]
21. Haddad F, Adams G, Bodell P, Baldwin KJJoAP. Isometric resistance exercise fails to counteract skeletal muscle atrophy processes during the initial stages of unloading. 2006. [DOI:10.1152/japplphysiol.01203.2005]
22. Jensky NE, Sims JK, Dieli-Conwright CM, Sattler FR, Rice JC, Schroeder ETJJos, et al. Exercise does not influence myostatin and follistatin mRNA expression in young women. 2010;24(2):522. [DOI:10.1519/JSC.0b013e3181c8664f]
23. Negaresh R, Ranjbar R, Baker JS, Habibi A, Mokhtarzade M, Gharibvand MM, et al. Skeletal muscle hypertrophy, insulin-like growth factor 1, myostatin and follistatin in healthy and sarcopenic elderly men: The effect of whole-body resistance training. 2019;10(1):29.
24. Willoughby DSJM, sports si, exercise. Effects of heavy resistance training on myostatin mRNA and protein expression. 2004;36(4):574-82. [DOI:10.1249/01.MSS.0000121952.71533.EA]
25. Welle S, Tawil R, Thornton CAJPo. Sex-related differences in gene expression in human skeletal muscle. 2008;3(1):e1385. [DOI:10.1371/journal.pone.0001385]
26. Philippe AG, Py G, Favier FB, Sanchez AM, Bonnieu A, Busso T, et al. Modeling the responses to resistance training in an animal experiment study. 2015;2015. [DOI:10.1155/2015/914860]
27. Thomas M, Langley B, Berry C, Sharma M, Kirk S, Bass J, et al. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. 2000;275(51):40235-43. [DOI:10.1074/jbc.M004356200]
28. Laurentino GC, Ugrinowitsch C, Roschel H, Aoki MS, Soares AG, Neves Jr M, et al. Strength training with blood flow restriction diminishes myostatin gene expression. 2012;44(3):406-12. [DOI:10.1249/MSS.0b013e318233b4bc]
29. Dutra DB, Bueno PG, Silva RN, Nakahara NH, Selistre‐Araújo HS, Nonaka KO, et al. Expression of myostatin, myostatin receptors and follistatin in diabetic rats submitted to exercise. 2012;39(5):417-22. [DOI:10.1111/j.1440-1681.2012.05690.x]
30. Roth SM, Martel GF, Ferrell RE, Metter EJ, Hurley BF, Rogers MAJEb, et al. Myostatin gene expression is reduced in humans with heavy-resistance strength training: a brief communication. 2003;228(6):706-9. [DOI:10.1177/153537020322800609]
31. Hulmi JJ, Ahtiainen JP, Kaasalainen T, PöLLANEN E, Hakkinen K, Alen M, et al. Postexercise myostatin and activin IIb mRNA levels: effects of strength training. 2007;39(2):289-97. [DOI:10.1249/01.mss.0000241650.15006.6e]
32. Schuelke M, Wagner KR, Stolz LE, Hübner C, Riebel T, Kömen W, et al. Myostatin mutation associated with gross muscle hypertrophy in a child. 2004;350(26):2682-8. [DOI:10.1056/NEJMoa040933]
33. Leiter JR, Peeler J, Anderson JEJM, nerve. Exercise‐induced muscle growth is muscle‐specific and age‐dependent. 2011;43(6):828-38. [DOI:10.1002/mus.21965]
34. Allen DL, Hittel DS, McPherron ACJM, sports si, exercise. Expression and function of myostatin in obesity, diabetes, and exercise adaptation. 2011;43(10):1828. [DOI:10.1249/MSS.0b013e3182178bb4]
35. Brandt C, Nielsen AR, Fischer CP, Hansen J, Pedersen BK, Plomgaard PJPO. Plasma and muscle myostatin in relation to type 2 diabetes. 2012;7(5):e37236. [DOI:10.1371/journal.pone.0037236]
36. Welle S, Bhatt K, Shah B, Thornton CAJEg. Insulin-like growth factor-1 and myostatin mRNA expression in muscle: comparison between 62-77 and 21-31 yr old men. 2002;37(6):833-9. [DOI:10.1016/S0531-5565(02)00025-6]
37. Zhao B, Wall RJ, Yang JJB, communications br. Transgenic expression of myostatin propeptide prevents diet-induced obesity and insulin resistance. 2005;337(1):248-55. [DOI:10.1016/j.bbrc.2005.09.044]
38. Suzuki ST, Zhao B, Yang JJB, communications br. Enhanced muscle by myostatin propeptide increases adipose tissue adiponectin, PPAR-α, and PPAR-γ expressions. 2008;369(2):767-73. [DOI:10.1016/j.bbrc.2008.02.092]
39. Antony N, Bass JJ, McMahon CD, Mitchell MDJAJoP-E, Metabolism. Myostatin regulates glucose uptake in BeWo cells. 2007. [DOI:10.1152/ajpendo.00331.2007]
40. Mitchell MD, Osepchook CC, Leung K-C, McMahon CD, Bass JJJTJoCE, Metabolism. Myostatin is a human placental product that regulates glucose uptake. 2006;91(4):1434-7. [DOI:10.1210/jc.2005-2361]
41. Moller DEJTiE, Metabolism. Potential role of TNF-α in the pathogenesis of insulin resistance and type 2 diabetes. 2000;11(6):212-7. [DOI:10.1016/S1043-2760(00)00272-1]
42. Guo T, Jou W, Chanturiya T, Portas J, Gavrilova O, McPherron ACJPo. Myostatin inhibition in muscle, but not adipose tissue, decreases fat mass and improves insulin sensitivity. 2009;4(3):e4937. [DOI:10.1371/journal.pone.0004937]
43. Tu P, Bhasin S, Hruz PW, Herbst KL, Castellani LW, Hua N, et al. Genetic disruption of myostatin reduces the development of proatherogenic dyslipidemia and atherogenic lesions in Ldlr null mice. 2009;58(8):1739-48. [DOI:10.2337/db09-0349]

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Volume 6, Issue 4 (Oct-Dec 2019) Back to browse issues page