[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 5، شماره 1 - ( 10-1396 ) ::
جلد 5 شماره 1 صفحات 47-54 برگشت به فهرست نسخه ها
Determination of Vitamin D3 in Fortified Iranian Flat Bread (Taftoon) Using High-Performance Liquid Chromatography
چکیده:   (749 مشاهده)

Background and Objectives: : Vitamin D3 measurement in food and fortified food matrixes is usually interrupted by several variables. This study aimed to elaborate a specific vitamin D3 quantification method for fortified Taftoon bread considering different influential variables in each step of measurement.
Materials and Methods: Dough was fortified with (4μg/100g) and the standard protocol No.13579 was followed with some modification in the extraction and determination steps.
Results: Addition of ethanol before adding potassium hydroxide and heating up the samples in tight containers in saponification step ceased clumping the extracts and helps it’s homogeneity. In extraction, distilled water addition following extraction with diethyl ether and Petroleum ether, adding ethanol at each step of washing and using SPE column result in an appropriate experimental extract. In the chromatography step, mobile phase substitution from methanol to 5:95 water, methanol mixture provided the method with more differentiation power. Using the above condition, commercial vitamin D3 was recovered from fortified Taftoon dough with 82-100% recovery and recovery of spiked vitamin D2 from dough and bread were ranged as 94 and 86.5%, respectively, Limit Of Detection(LOD) and Limit Of Quantification(LOQ) of the method were 0.04 and 0.1mg L-1 respectively, which equaled 5μg/Kg and 10μg/Kg. True retention for the added commercial vitamin D3 in Taftoon bread was obtained as 83.4% which increased to 92% after six months storage in freezer.
Conclusions: Results of this study can be used to specify a protocol for vitamin D3 measurement in fortified bread samples. Modified experiment which is projected in this study would specify the general protocol of vitamin D3 measurement that was previously proposed in the national standard of Iran for developing a new and practical national standard document.

متن کامل [PDF 228 kb]   (295 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: ۱۳۹۶/۵/۳۱ | پذیرش: ۱۳۹۶/۱۱/۱۱ | انتشار: ۱۳۹۶/۱۱/۱۱
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA code



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tabibian M, Sadeghi M, Mohtadinia J, Babashahi M, Mirlohi M. Determination of Vitamin D3 in Fortified Iranian Flat Bread (Taftoon) Using High-Performance Liquid Chromatography. Nutr Food Sci Res. 2018; 5 (1) :47-54
URL: http://nfsr.sbmu.ac.ir/article-1-261-fa.html

Determination of Vitamin D3 in Fortified Iranian Flat Bread (Taftoon) Using High-Performance Liquid Chromatography. 1. 1396; 5 (1) :47-54

URL: http://nfsr.sbmu.ac.ir/article-1-261-fa.html



دوره 5، شماره 1 - ( 10-1396 ) برگشت به فهرست نسخه ها
Nutrition and Food Sciences Research
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3781